Combined exposure to hypoxia and nanoplastics leads to negative synergistic oxidative stress-mediated effects in the water flea Daphnia magna.

Autor: Lee Y; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea., Kim DH; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea., Lee JS; School of Pharmacy, Sungkyunkwan University, Suwon 16419, South Korea., Kim HS; School of Pharmacy, Sungkyunkwan University, Suwon 16419, South Korea., Maszczyk P; Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Wang M; Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment & Ecology, Xiamen University, Xiamen 361102, China., Yang Z; Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, China., Wang DZ; State Key Laboratory of Marine Environmental Science, College of the Environment & Ecology, Xiamen University, Xiamen 361102, China., Lee JS; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address: jslee2@skku.edu.
Jazyk: angličtina
Zdroj: Marine pollution bulletin [Mar Pollut Bull] 2024 May; Vol. 202, pp. 116306. Date of Electronic Publication: 2024 Apr 03.
DOI: 10.1016/j.marpolbul.2024.116306
Abstrakt: In this study, we investigated the combined effects of hypoxia and NPs on the water flea Daphnia magna, a keystone species in freshwater environments. To measure and understand the oxidative stress responses, we used acute toxicity tests, fluorescence microscopy, enzymatic assays, Western blot analyses, and Ingenuity Pathway Analysis. Our findings demonstrate that hypoxia and NPs exhibit a negative synergy that increases oxidative stress, as indicated by heightened levels of reactive oxygen species and antioxidant enzyme activity. These effects lead to more severe reproductive and growth impairments in D. magna compared to a single-stressor exposure. In this work, molecular investigations revealed complex pathway activations involving HIF-1α, NF-κB, and mitogen-activated protein kinase, illustrating the intricate molecular dynamics that can occur in combined stress conditions. The results underscore the amplified physiological impacts of combined environmental stressors and highlight the need for integrated strategies in the management of aquatic ecosystems.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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