A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster .

Autor:	Sang J; Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of Korea., Dhakal S; Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of Korea., Shrestha B; Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of Korea., Nath DK; Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of Korea., Kim Y; Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of Korea., Ganguly A; Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, United States., Montell C; Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, United States., Lee Y; Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of Korea.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2024 Apr 04; Vol. 12. Date of Electronic Publication: 2024 Apr 04.
DOI:	10.7554/eLife.93464
Abstrakt:	Salt (NaCl), is an essential nutrient for survival, while excessive salt can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in the pharynx are critical gatekeepers impacting the decision to accept or reject a food. Currently, our understanding of the mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high salt are rudimentary. Here, we found that a member of the ionotropic receptor family, Ir60b , is expressed exclusively in a pair of GRNs activated by high salt. Using a two-way choice assay (DrosoX) to measure ingestion volume, we demonstrate that IR60b and two co-receptors IR25a and IR76b are required to prevent high salt consumption. Mutants lacking external taste organs but retaining the internal taste organs in the pharynx exhibit much higher salt avoidance than flies with all taste organs but missing the three IRs. Our findings highlight the vital role for IRs in a pharyngeal GRN to control ingestion of high salt. Competing Interests: JS, SD, BS, DN, YK, AG, CM, YL No competing interests declared (© 2023, Sang, Dhakal et al.)
Databáze:	MEDLINE
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