Intestinal Colonization by Campylobacter jejuni , Clostridium difficile , and Clostridium perfringens among Commensal Rattus norvegicus in the Urban Areas of Tehran, Iran.

Autor: Azimi T; Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Nasrollahian S; Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Sabour S; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran., Mosadegh M; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Hadi N; Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Azimi L; Pediatric Infections Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Fallah F; Pediatric Infections Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Pourmand MR; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2024 Mar 27; Vol. 2024, pp. 2929315. Date of Electronic Publication: 2024 Mar 27 (Print Publication: 2024).
DOI: 10.1155/2024/2929315
Abstrakt: Background: Rattus norvegicus ( R. norvegicus ) population plays a significant role in the spread of numerous diseases in urban environments. The present study is aimed at investigating the presence of Campylobacter jejuni ( C. jejuni ), C. coli , Clostridium difficile ( C. difficile ), C. difficile toxigenic, and C. perfringens in R. norvegicus captured from urban areas of Tehran, Iran.
Methods: From October 2021 to October 2022, 100 urban rats were trapped in 5 different districts of Tehran, Iran. The genomic DNA was extracted from fecal samples, and the presence of C. jejuni , C. coli , C. perfringens , and C. difficile species was evaluated using PCR assay. Moreover, PCR was used to assess the toxicity of C. difficile isolates.
Results: Overall, 30% ( n = 30/100) of fecal samples were positive for zoonotic pathogens. Based on the PCR on hippuricase ( hipO ), glycine ( gly ), CIDIF , and phospholipase C ( plc ) genes, C. perfringens and C. difficile were isolated from 18.2% ( n = 14/77) and 5.2% ( n = 4/77) of male rats. The highest frequency of C. perfringens and C . jejuni was 25% ( n = 5/20) related to the south of Tehran. Toxigenic C. difficile was not detected in all regions.
Conclusion: According to the findings, rats are the main reservoirs for diseases. Therefore, rodent control coupled with the implementation of surveillance systems should be prioritized for urban health.
Competing Interests: The authors declare that they have no competing interests.
(Copyright © 2024 Taher Azimi et al.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje