Analgesic effect of a cholinergic agonist (carbachol) in a sural nerve ligation-induced hypersensitivity mouse model.

Autor:	Kato N; Department of Pharmacology, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima, Japan., Kambe T; Department of Medicinal Pharmacology, Showa Pharmaceutical University, Machida, Tokyo, Japan., Chiba T; Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University, Saitama, Japan., Taguchi K; Department of Pharmacology, ShowaPharmaceutical University, Machida, Tokyo, Japan., Abe K; Department of Pharmacology, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima, Japan.; Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University, Saitama, Japan.
Jazyk:	angličtina
Zdroj:	Neurological research [Neurol Res] 2024 Jun; Vol. 46 (6), pp. 505-515. Date of Electronic Publication: 2024 Apr 03.
DOI:	10.1080/01616412.2024.2337512
Abstrakt:	Objectives: Neuropathic pain is characterized by long-lasting, intractable pain. Sciatic nerve ligation is often used as an animal model of neuropathic pain, and the spared nerve injury (SNI) model, in which the common peroneal nerve (CPN) and tibial nerve (TN) are ligated, is widely used. In the present study, we evaluated the analgesic effect of a cholinergic agonist, carbachol, on a neuropathic pain model prepared by sural nerve (SN) ligation in mice. Methods: The SN was tightly ligated as a branch of the sciatic nerve. Mechanical and thermal allodynia, and hyperalgesia were assessed using von Frey filaments and heat from a hot plate. The analgesic effects of intracerebroventricularly-administered morphine and carbachol were compared. Results: SN ligation resulted in a significant decrease in pain threshold for mechanical stimulation 1 day after ligation. In response to thermal stimulation, allodynia was observed at 50°C and hyperalgesia at 53 and 56°C 3 days after ligation. Content of thiobarbituric acid reactive substances (TBARS) in the spinal cord increased significantly at 6 and 12 h after ligation. Acetylcholine content of the spinal cord also increased at 5 and 7 days after ligation. Intracerebroventricular administration of carbachol at 7 days after ligation produced a marked analgesic effect against mechanical and thermal stimuli, which was stronger and longer-lasting than morphine at all experimental time points. Conclusion: These findings suggest that cholinergic nerves are involved in allodynia and hyperalgesia of the SN ligation neuropathic pain model.
Databáze:	MEDLINE
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