Unveiling the distribution characteristics of rpf-like genes and indigenous resuscitation promoting factor production in PCB-contaminated soils.

Autor: Shi J; College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China., Zhou X; College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China., Zhang S; The Management Center of Wuyanling National Natural Reserve in Zhejiang, Wenzhou, 325500, China., Sun F; College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China., Shen C; Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China., Su X; College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China. Electronic address: purple@zjnu.cn.
Jazyk: angličtina
Zdroj: Journal of environmental management [J Environ Manage] 2024 Apr; Vol. 357, pp. 120803. Date of Electronic Publication: 2024 Apr 03.
DOI: 10.1016/j.jenvman.2024.120803
Abstrakt: Resuscitation promoting factors (Rpfs), known for their anti-dormancy cytokine properties, have been extensively investigated in the medical field. Although the Rpf from Micrococcus luteus has been successfully utilized to resuscitate and stimulate microbial populations for the degradation of polychlorinated biphenyls (PCBs), the presence of indigenous Rpf homologs in PCB-contaminated soils has not been established. In this study, the distribution characteristics of rpf-like genes and indigenous strain capable of producing Rpf in PCB-contaminated soils were explored. The results revealed the widespread presence of Rpf-like domains and their associated genes, particularly in close association with heavy metals and PCBs. The rpf-like genes were predominantly found in Proteobacteria and displayed a positive correlation with genes involved in PCB degradation and viable but non-culturable (VBNC) formation. Notably, the recombinant Rpf-Ac protein derived from the indigenous strain Achromobacter sp. HR2 exhibited muralytic activity and demonstrated significant efficacy in resuscitating the growth of VBNC cells, while also stimulating the growth of normal cells. These findings shed light on the prevalent presence of Rpf homologs in PCB-contaminated soils and their potential to resuscitate functional populations in the VBNC state, thereby enhancing in situ bioremediation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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