Development of a latex agglutination test based on VH antibody fragment for detection of Streptococcus suis serotype 2.

Autor: Chidkoksung K; The Medical Microbiology Program, Graduate School, Chulalongkorn University, Bangkok, Thailand., Parakasikron N; The Medical Microbiology Program, Graduate School, Chulalongkorn University, Bangkok, Thailand., Nuanualsuwan S; Department of Veterinary Public Health, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.; Food Risk Hub, Research Unit of Chulalongkorn University, Bangkok, Thailand., Khantasup K; Food Risk Hub, Research Unit of Chulalongkorn University, Bangkok, Thailand.; Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Apr 03; Vol. 19 (4), pp. e0299691. Date of Electronic Publication: 2024 Apr 03 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0299691
Abstrakt: Streptococcus suis serotype 2 (SS2) is an important porcine pathogen that causes diseases in both swine and human. For rapid SS2 identification, a novel latex agglutination test (LAT) based on heavy-chain variable domain antibody (VH) was developed. Firstly, the soluble 47B3 VH antibody fragment from a phage display library, in which cysteine residues were engineered at the C-terminus, was expressed in Escherichia coli. The purified protein was then gently reduced to form monomeric soluble 47B3 VH subsequently used to coat with latex beads by means of site-specific conjugation. The resulting VH-coated beads gave a good agglutination reaction with SS2. The LAT was able to distinguish S. suis serotype 2 from serotype 1/2, which shares some common sugar residues, and showed no cross-reaction with other serotypes of S. suis or other related bacteria. The detection sensitivity was found to be as high as 1.85x106 cells. The LAT was stable at 4°C for at least six months without loss of activity. To the best of our knowledge, this is the first LAT based on a VH antibody fragment that can be considered as an alternative for conventional antibody-based LAT where VHs are the most favored recombinant antibody.
Competing Interests: No. The authors have declared that no competing interests exist.
(Copyright: © 2024 Chidkoksung et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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