Mitochondrial Calcium Uniporter (MCU) is Involved in an Ischemic Postconditioning Effect Against Ischemic Reperfusion Brain Injury in Mice.

Autor: Sasaki H; Department of Neurosurgery, Nara Medical University, Shijo-Cho 840, Kashihara City, Nara, 634-8522, Japan., Nakagawa I; Department of Neurosurgery, Nara Medical University, Shijo-Cho 840, Kashihara City, Nara, 634-8522, Japan. nakagawa@naramed-u.ac.jp., Furuta T; Department of Neurosurgery, Nara Medical University, Shijo-Cho 840, Kashihara City, Nara, 634-8522, Japan., Yokoyama S; Department of Neurosurgery, Nara Medical University, Shijo-Cho 840, Kashihara City, Nara, 634-8522, Japan., Morisaki Y; Department of Neurosurgery, Nara Medical University, Shijo-Cho 840, Kashihara City, Nara, 634-8522, Japan., Saito Y; Department of Neurophysiology, Nara Medical University, Shijo-Cho 840, Kashihara City, Nara, 634-8522, Japan., Nakase H; Department of Neurosurgery, Nara Medical University, Shijo-Cho 840, Kashihara City, Nara, 634-8522, Japan.
Jazyk: angličtina
Zdroj: Cellular and molecular neurobiology [Cell Mol Neurobiol] 2024 Apr 03; Vol. 44 (1), pp. 32. Date of Electronic Publication: 2024 Apr 03.
DOI: 10.1007/s10571-024-01464-7
Abstrakt: The phenomenon of ischemic postconditioning (PostC) is known to be neuroprotective against ischemic reperfusion (I/R) injury. One of the key processes in PostC is the opening of the mitochondrial ATP-dependent potassium (mito-K ATP ) channel and depolarization of the mitochondrial membrane, triggering the release of calcium ions from mitochondria through low-conductance opening of the mitochondrial permeability transition pore. Mitochondrial calcium uniporter (MCU) is known as a highly sensitive transporter for the uptake of Ca 2+ present on the inner mitochondrial membrane. The MCU has attracted attention as a new target for treatment in diseases, such as neurodegenerative diseases, cancer, and ischemic stroke. We considered that the MCU may be involved in PostC and trigger its mechanisms. This research used the whole-cell patch-clamp technique on hippocampal CA1 pyramidal cells from C57BL mice and measured changes in spontaneous excitatory post-synaptic currents (sEPSCs), intracellular Ca 2+ concentration, mitochondrial membrane potential, and N-methyl-D-aspartate receptor (NMDAR) currents under inhibition of MCU by ruthenium red 265 (Ru265) in PostC. Inhibition of MCU increased the occurrence of sEPSCs (p = 0.014), NMDAR currents (p < 0.001), intracellular Ca 2+ concentration (p < 0.001), and dead cells (p < 0.001) significantly after reperfusion, reflecting removal of the neuroprotective effects in PostC. Moreover, mitochondrial depolarization in PostC with Ru265 was weakened, compared to PostC (p = 0.004). These results suggest that MCU affects mitochondrial depolarization in PostC to suppress NMDAR over-activation and prevent elevation of intracellular Ca 2+ concentrations against I/R injury.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: