Synergistic Interaction Effect of Artemisia cina n-hexane Extract and Tagetes lucida Ethyl Acetate Extract on Haemonchus Contortus.

Autor: Santiago-Figueroa I; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carr. Cuautitlán- Teoloyucan Km 2.5, Col. San Sebastián Xhala, CP 54714, Cuautitlán, México., González-Cortazar M; Centro de Investigación Biomédica Del Sur, Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, 62790, Xochitepec, CP, México., Estrada-Flores JG; Instituto de Ciencias Agropecuarias y Rurales (ICAR), Universidad Autónoma del Estado de México (UAEM), Estado de México, Campus UAEM El Cerrillo, El Cerrillo Piedras Blancas, 50090, Toluca, México., Cuéllar-Ordaz JA; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carr. Cuautitlán- Teoloyucan Km 2.5, Col. San Sebastián Xhala, CP 54714, Cuautitlán, México., López-Arellano ME; Centro de Investigación Disciplinaria en Salud Animal e Inocuidad, Agrícolas y Pecuarias (INIFAP), Instituto Nacional de Investigaciones Forestales, Carr. Fed. Cuernavaca-Cuautla No. 8534, CP 62550, Jiutepec, México., González-Reyes FJ; Universidad Autónoma Chapingo, Carr. México-Texcoco Km 38.5, C.P. 56230, Chapingo, Estado de México, México., Olmedo-Juárez A; Centro de Investigación Disciplinaria en Salud Animal e Inocuidad, Agrícolas y Pecuarias (INIFAP), Instituto Nacional de Investigaciones Forestales, Carr. Fed. Cuernavaca-Cuautla No. 8534, CP 62550, Jiutepec, México. aolmedoj@gmail.com., Higuera-Piedrahita RI; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carr. Cuautitlán- Teoloyucan Km 2.5, Col. San Sebastián Xhala, CP 54714, Cuautitlán, México. rhiguera05@comunidad.unam.mx.
Jazyk: angličtina
Zdroj: Acta parasitologica [Acta Parasitol] 2024 Jun; Vol. 69 (2), pp. 1132-1140. Date of Electronic Publication: 2024 Apr 03.
DOI: 10.1007/s11686-024-00839-6
Abstrakt: Purpose: We analysed the possible synergistic activity among active extracts from Artemisia cina and Tagetes lucida combinations on Haemonchus contortus, a nematode parasitising sheep.
Methods: The work was carried out in vitro on eggs and infective larvae (L3) of H. contortus. The results were analysed with SAS 9.1, applying the ANOVA and Tukey test, and the lethal concentration (LC) values LC50 and LC90 were determined with regression analysis, employing Proc Probit of SAS 9.1. Additionally, the lethal concentration (LC) was calculated with LC 50 and LC 90 to determine the synergistic effect.
Results: The results demonstrated a high efficacy of the two plants studied on both nematode eggs and L3 larvae as well as of their combinations. The highest egg hatching inhibition was obtained with a 50/50 combination, and the best larvae mortality was obtained with 25% A. cina and 75% T. lucida at 10 mg/mL. Additionally, this combination showed a synergistic effect.
Conclusion: The two plant species studied here can be applied as natural anthelmintic alternatives due to their high bioactive effect and synergistic response.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: