Spectral changes of light-harvesting complex 2 lacking B800 bacteriochlorophyll a under neutral pH conditions.

Autor: Kawato S; Faculty of Science and Engineering, Kindai University, Higashi-Osaka, Osaka, 577-8502, Japan., Sato S; Faculty of Science and Engineering, Kindai University, Higashi-Osaka, Osaka, 577-8502, Japan., Kitoh-Nishioka H; Faculty of Science and Engineering, Kindai University, Higashi-Osaka, Osaka, 577-8502, Japan., Saga Y; Faculty of Science and Engineering, Kindai University, Higashi-Osaka, Osaka, 577-8502, Japan. saga@chem.kindai.ac.jp.
Jazyk: angličtina
Zdroj: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2024 May; Vol. 23 (5), pp. 871-879. Date of Electronic Publication: 2024 Apr 02.
DOI: 10.1007/s43630-024-00560-3
Abstrakt: Exchange of B800 bacteriochlorophyll (BChl) a in light-harvesting complex 2 (LH2) is promising for a better understanding of the mechanism on intracomplex excitation energy transfer of this protein. Structural and spectroscopic properties of LH2 lacking B800 BChl a (B800-depleted LH2), which is an important intermediate protein in the B800 exchange, will be useful to tackle the energy transfer mechanism in LH2 by the B800 exchange strategy. In this study, we report a unique spectral change of B800-depleted LH2, in which the Q y absorption band of B800 BChl a is automatically recovered under neutral pH conditions. This spectral change was facilitated by factors for destabilization of LH2, namely, a detergent, lauryl dimethylamine N-oxide, and an increase in temperature. Spectral analyses in the preparation of an LH2 variant denoted as B800-recovered LH2 indicated that most BChl a that was released by decomposition of part of B800-depleted LH2 was a source of the production of B800-recovered LH2. Characterization of purified B800-recovered LH2 demonstrated that its spectroscopic and structural features was quite similar to those of native LH2. The current results indicate that the recovery of the B800 Q y band of B800-depleted LH2 originates from the combination of decomposition of part of B800-depleted LH2 and in situ reconstitution of BChl a into the B800 binding pockets of residual B800-depleted LH2, resulting in the formation of stable B800-recovered LH2.
(© 2024. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.)
Databáze: MEDLINE
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