RNase H-dependent DNA thresholder modulated by cancer marker concentration.

Autor: Nour MAY; Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute ITMO University, Saint-Petersburg, Russia, 9 Lomonosova Str., St. Petersburg, 191002, Russian Federation. eldeeb@scamt-itmo.ru., Drozd VS; Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute ITMO University, Saint-Petersburg, Russia, 9 Lomonosova Str., St. Petersburg, 191002, Russian Federation. eldeeb@scamt-itmo.ru., Lemeshko EA; Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute ITMO University, Saint-Petersburg, Russia, 9 Lomonosova Str., St. Petersburg, 191002, Russian Federation. eldeeb@scamt-itmo.ru., Tafran L; Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute ITMO University, Saint-Petersburg, Russia, 9 Lomonosova Str., St. Petersburg, 191002, Russian Federation. eldeeb@scamt-itmo.ru., Salimova AA; Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute ITMO University, Saint-Petersburg, Russia, 9 Lomonosova Str., St. Petersburg, 191002, Russian Federation. eldeeb@scamt-itmo.ru., Kulikova AV; Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute ITMO University, Saint-Petersburg, Russia, 9 Lomonosova Str., St. Petersburg, 191002, Russian Federation. eldeeb@scamt-itmo.ru., Eldeeb AA; Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute ITMO University, Saint-Petersburg, Russia, 9 Lomonosova Str., St. Petersburg, 191002, Russian Federation. eldeeb@scamt-itmo.ru.
Jazyk: angličtina
Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2024 Apr 18; Vol. 60 (33), pp. 4427-4430. Date of Electronic Publication: 2024 Apr 18.
DOI: 10.1039/d4cc00495g
Abstrakt: Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the level of oncogene expression followed by silencing of a targeted gene.
Databáze: MEDLINE
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