Comparative analysis of human tear fluid and aqueous humor proteomes.

Autor: Beisel A; Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: abeisel@augusta.edu., Jones G; Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: garjones@augusta.edu., Glass J; Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: josglass@augusta.edu., Lee TJ; Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: talee@augusta.edu., Töteberg-Harms M; Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: mtoetebergharms@augusta.edu., Estes A; Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: aestes@augusta.edu., Ulrich L; Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: lulrich@augusta.edu., Bollinger K; Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: kbollinger@augusta.edu., Sharma S; Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA; Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: shsharma@augusta.edu., Sharma A; Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA; Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA; Department of Population Health Sciences, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA. Electronic address: assharma@augusta.edu.
Jazyk: angličtina
Zdroj: The ocular surface [Ocul Surf] 2024 Jul; Vol. 33, pp. 16-22. Date of Electronic Publication: 2024 Mar 30.
DOI: 10.1016/j.jtos.2024.03.011
Abstrakt: Purpose: Technological advancements allowing for the analysis of low-volume samples have led to the investigation of human tear fluid and aqueous humor (AH) as potential biomarker sources. However, acquiring AH samples poses significant challenges, making human tear fluid a more accessible alternative. This study aims to compare the protein compositions of these two biofluids to evaluate their suitability for biomarker discovery.
Methods: Paired tear and AH samples were collected from 20 patients undergoing cataract surgery. Tear samples were collected using Schirmer strips prior to surgery, and AH samples were collected from the anterior chamber immediately after corneal incision. Proteins were extracted and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Results: A total of 481 proteins were identified in greater than 50% of the tear samples, and 191 proteins were detected in greater than 50% of the AH samples. Of these proteins, 82 were found to be common between the two biofluids, with ALB, LTF, TF, LCN1, and IGKC being the most abundant.
Conclusion: Although tear fluid and the AH are functionally independent and physically separated, many of the proteins detected in AH were also detected in tears. This direct comparison of the proteomic content of tear fluid and AH may aid in further investigation of tear fluid as a source of readily accessible biomarkers for various human diseases.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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