Multifunctional host-defense peptides isolated from skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae).

Autor: Conlon JM; Diabetes Research Centre, School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK. Electronic address: jmconlon1@gmail.com., Sridhar A; Diabetes Research Centre, School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK., Khan D; Diabetes Research Centre, School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK., Cunning TS; Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK., Delaney JJ; Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK., Taggart MG; Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK., Ternan NG; Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK., Leprince J; Université Rouen Normandie, Inserm, NorDiC UMR 1239, HeRacLeS, US 51, PRIMACEN, F-76000, Rouen, France., Coquet L; CNRS UAR2026 HeRacLeS-PISSARO, CNRS UMR 6270 PBS, Université Rouen Normandie, 76821, Mont-Saint-Aignan, France., Jouenne T; CNRS UAR2026 HeRacLeS-PISSARO, CNRS UMR 6270 PBS, Université Rouen Normandie, 76821, Mont-Saint-Aignan, France., Attoub S; Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, 15551, Al Ain, United Arab Emirates., Mechkarska M; Department of Life Sciences, Faculty of Science and Technology, University of The West Indies, St. Augustine Campus, Trinidad and Tobago.
Jazyk: angličtina
Zdroj: Biochimie [Biochimie] 2024 Aug; Vol. 223, pp. 23-30. Date of Electronic Publication: 2024 Mar 30.
DOI: 10.1016/j.biochi.2024.03.012
Abstrakt: Five host-defense peptides (figainin 2PL, hylin PL, raniseptin PL, plasticin PL, and peptide YL) were isolated from norepinephrine-stimulated skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae) collected in Trinidad. Raniseptin PL (GVFDTVKKIGKAVGKFALGVAKNYLNS.NH 2 ) and figainin 2PL (FLGTVLKLGKAIAKTVVPMLTNAMQPKQ. NH 2 ) showed potent and rapid bactericidal activity against a range of clinically relevant Gram-positive and Gram-negative ESKAPE  +  pathogens and Clostridioides difficile. The peptides also showed potent cytotoxic activity (LC 50 values < 30 μM) against A549, MDA-MB-231 and HT29 human tumor-derived cell lines but appreciably lower hemolytic activity against mouse erythrocytes (LC 50  = 262 ± 14 μM for raniseptin PL and 157 ± 16 μM for figainin 2PL). Hylin PL (FLGLIPALAGAIGNLIK.NH 2 ) showed relatively weak activity against microorganisms but was more hemolytic. The glycine-leucine-rich peptide with structural similarity to the plasticins (GLLSTVGGLVGGLLNNLGL.NH 2 ) and the non-cytotoxic peptide YL (YVPGVIESLL.NH 2 ) lacked antimicrobial and cytotoxic activities. Hylin PL, raniseptinPL and peptide YL stimulated the rate of release of insulin from BRIN-BD11 clonal β-cells at concentrations ≥100 nM. Peptide YL was the most effective (2.3-fold increase compared with basal rate at 1 μM concentration) and may represent a template for the design of a new class of incretin-based anti-diabetic drugs.
Competing Interests: Declaration of competing interest Authors have no competing interests to declare.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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