A pan-genomic assessment: Delving into the genome of the marine epiphyte Bacillus altitudinis strain 19_A and other very close Bacillus strains from multiple environments.
| Autor: | Comba-González NB; Biological and Environmental Sciences Area, Jorge Tadeo Lozano University, Colombia., Chaves-Moreno D; Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany., Santamaría-Vanegas J; Biological and Environmental Sciences Area, Jorge Tadeo Lozano University, Colombia., Montoya-Castaño D; Bioprocesses and Bioprospecting Group, Biotechnology Institute, Universidad Nacional de Colombia, Colombia. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2024 Mar 20; Vol. 10 (7), pp. e27820. Date of Electronic Publication: 2024 Mar 20 (Print Publication: 2024). |
| DOI: | 10.1016/j.heliyon.2024.e27820 |
| Abstrakt: | Marine macroalgae are the habitat of epiphytic bacteria and provide several conditions for a beneficial biological interaction to thrive. Although Bacillus is one of the most abundant epiphytic genera, genomic information on marine macroalgae-associated Bacillus species remains scarce. In this study, we further investigated our previously published genome of the epiphytic strain Bacillus altitudinis 19_A to find features that could be translated to potential metabolites produced by this microorganism, as well as genes that play a role in its interaction with its macroalgal host. To achieve this goal, we performed a pan-genome analysis of Bacillus sp. and a codon bias assessment, including the genome of the strain Bacillus altitudinis 19_A and 29 complete genome sequences of closely related Bacillus strains isolated from soil, marine environments, plants, extreme environments, air, and food. This genomic analysis revealed that Bacillus altitudinis 19_A possessed unique genes encoding proteins involved in horizontal gene transfer, DNA repair, transcriptional regulation, and bacteriocin biosynthesis. In this comparative analysis, codon bias was not associated with the habitat of the strains studied. Some accessory genes were identified in the Bacillus altitudinis 19_A genome that could be related to its epiphytic lifestyle, as well as gene clusters for the biosynthesis of a sporulation-killing factor and a bacteriocin, showing their potential as a source of antimicrobial peptides. Our results provide a comprehensive view of the Bacillus altitudinis 19_A genome to understand its adaptation to the marine environment and its potential as a producer of bioactive compounds. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.) |
| Databáze: | MEDLINE |
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