Host proteins interact with viral elements and affect the life cycle of highly pathogenic avian influenza A virus H7N9.

Autor:	Yu DS; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China.; Department of Infectious Disease, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, PR China., Wu XX; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China., Weng TH; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China., Cheng LF; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China., Liu FM; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China., Wu HB; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China.; Department of Infectious Disease, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, PR China., Lu XY; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China., Wu NP; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China.; Jinan Microecological Biomedicine Shandong Laboratory, Jinan, 250021, PR China., Sun SL; Department of Infectious Disease, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, PR China., Yao HP; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, 310003, PR China.; Jinan Microecological Biomedicine Shandong Laboratory, Jinan, 250021, PR China.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Mar 19; Vol. 10 (7), pp. e28218. Date of Electronic Publication: 2024 Mar 19 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e28218
Abstrakt:	Host-virus interactions can significantly impact the viral life cycle and pathogenesis; however, our understanding of the specific host factors involved in highly pathogenic avian influenza A virus H7N9 (HPAI H7N9) infection is currently restricted. Herein, we designed and synthesized 65 small interfering RNAs targeting host genes potentially associated with various aspects of RNA virus life cycles. Afterward, HPAI H7N9 viruses were isolated and RNA interference was used to screen for host factors likely to be involved in the life cycle of HPAI H7N9. Moreover, the research entailed assessing the associations between host proteins and HPAI H7N9 proteins. Twelve key host proteins were identified: Annexin A (ANXA)2, ANXA5, adaptor related protein complex 2 subunit sigma 1 (AP2S1), adaptor related protein complex 3 subunit sigma 1 (AP3S1), ATP synthase F1 subunit alpha (ATP5A1), COPI coat complex subunit alpha (COP)A, COPG1, heat shock protein family A (Hsp70) member 1A (HSPA)1A, HSPA8, heat shock protein 90 alpha family class A member 1 (HSP90AA1), RAB11B, and RAB18. Co-immunoprecipitation revealed intricate interactions between viral proteins (hemagglutinin, matrix 1 protein, neuraminidase, nucleoprotein, polymerase basic 1, and polymerase basic 2) and these host proteins, presumably playing a crucial role in modulating the life cycle of HPAI H7N9. Notably, ANXA5, AP2S1, AP3S1, ATP5A1, HSP90A1, and RAB18, were identified as novel interactors with HPAI H7N9 proteins rather than other influenza A viruses (IAVs). These findings underscore the significance of host-viral protein interactions in shaping the dynamics of HPAI H7N9 infection, while highlighting subtle variations compared with other IAVs. Deeper understanding of these interactions holds promise to advance disease treatment and prevention strategies. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
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