Availability of individual proteins for quantitative analysis in postmortem brains preserved in two different brain banks.

Autor: Nagaoka A; Department of Psychiatry, Tohoku University Hospital, Sendai, Japan.; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan., Hino M; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.; Department of Disaster Psychiatry, International Research Institute of Disaster Science, Tohoku University, Sendai, Japan., Izumi R; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan., Shishido R; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan., Ishibashi M; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan., Hatano M; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan., Sainouchi M; Department of Pathology, Brain Research Institute, Niigata University, Niigata, Japan., Kakita A; Department of Pathology, Brain Research Institute, Niigata University, Niigata, Japan., Tomita H; Department of Psychiatry, Tohoku University Hospital, Sendai, Japan.; Department of Disaster Psychiatry, International Research Institute of Disaster Science, Tohoku University, Sendai, Japan.; Department of Psychiatry, Graduate School of Medicine, Tohoku University, Sendai, Japan., Kunii Y; Department of Psychiatry, Tohoku University Hospital, Sendai, Japan.; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.; Department of Disaster Psychiatry, International Research Institute of Disaster Science, Tohoku University, Sendai, Japan.
Jazyk: angličtina
Zdroj: Neuropsychopharmacology reports [Neuropsychopharmacol Rep] 2024 Jun; Vol. 44 (2), pp. 399-409. Date of Electronic Publication: 2024 Apr 01.
DOI: 10.1002/npr2.12430
Abstrakt: Aim: Postmortem brain research is necessary for elucidating the pathology of schizophrenia; an increasing number of studies require a combination of suitable tissue samples preserved at multiple brain banks. In this study, we examined whether a comparative study of protein expression levels can be conducted using postmortem brain samples preserved in different facilities.
Methods: We compared the demographic factors of postmortem brain samples preserved in two institutions and measured and compared the expression levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glial fibrillary acidic protein (GFAP) in the prefrontal cortex and superior temporal gyrus. GAPDH is generally used as a loading control for western blotting, and GFAP is considered as an astrocyte marker in the brain.
Results: We found significant differences between the two institutions in postmortem interval, age at death, and preservation time. To reduce the effects of these differences on our measurements, the parameters were set as covariates in our analyses of covariance. Subsequently, no differences in GAPDH and GFAP expression were found between institutions.
Conclusions: When studies are conducted using brain samples preserved in different brain banks, differences in demographic factors should be carefully considered and taken into account by statistical methods to minimize their impact as much as possible. Since there was no significant difference in the protein expression levels of GAPDH and GFAP in either region between the two institutions that preserved the postmortem brains, we concluded that it is possible to perform protein quantitative analysis assuming that there is no effect of difference between two institutions.
(© 2024 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.)
Databáze: MEDLINE
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