Radiotherapy protocols for mouse cancer model.

Autor:	Garate-Soraluze E; Program of Immunology and Immunotherapy, Center for Applied Medical Research (CIMA), Pamplona, Spain., Marco-Sanz J; Program of Solid Tumors, Center for Applied Medical Research (CIMA), Pamplona, Spain; Department of Pediatrics, University of Navarra Clinic, Pamplona, Spain; Navarra Institute for Health Research (IDISNA), Pamplona, Spain., Serrano-Mendioroz I; Program of Immunology and Immunotherapy, Center for Applied Medical Research (CIMA), Pamplona, Spain., Marrodán L; Program of Solid Tumors, Center for Applied Medical Research (CIMA), Pamplona, Spain; Navarra Institute for Health Research (IDISNA), Pamplona, Spain., Fernandez-Rubio L; Program of Immunology and Immunotherapy, Center for Applied Medical Research (CIMA), Pamplona, Spain., Labiano S; Program of Solid Tumors, Center for Applied Medical Research (CIMA), Pamplona, Spain; Department of Pediatrics, University of Navarra Clinic, Pamplona, Spain; Navarra Institute for Health Research (IDISNA), Pamplona, Spain., Rodríguez-Ruiz ME; Program of Immunology and Immunotherapy, Center for Applied Medical Research (CIMA), Pamplona, Spain; Navarra Institute for Health Research (IDISNA), Pamplona, Spain; Department of Radiation Oncology, University of Navarra Clinic, Pamplona, Spain; Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Madrid, Spain. Electronic address: mrruiz@unav.es.
Jazyk:	angličtina
Zdroj:	Methods in cell biology [Methods Cell Biol] 2024; Vol. 185, pp. 99-113. Date of Electronic Publication: 2024 Mar 23.
DOI:	10.1016/bs.mcb.2024.02.007
Abstrakt:	Radiotherapy is a crucial treatment modality for cancer patients, with approximately 60% of individuals undergoing ionizing radiation as part of their disease management. In recent years, there has been a growing trend toward minimizing irradiation fields through the use of image-guided dosimetry and innovative technologies. These advancements allow for selective irradiation, delivering higher local doses while reducing the number of treatment sessions. Consequently, computer-assisted methods have significantly enhanced the effectiveness of radiotherapy in the curative and palliative treatment of various cancers. Although radiation therapy alone can effectively achieve local control in some cancer types, it may not be sufficient for others. As a result, further preclinical research is necessary to explore novel approaches including new schedules of radiotherapy treatments. Unfortunately, there is a concerning lack of correlation between clinical outcomes and experiments conducted on mouse models. We hypothesize that this disparity arises from the differences in irradiation strategies employed in preclinical studies compared to those used in clinical practice, which ultimately affects the translatability of findings to patients. In this study, we present two comprehensive radiotherapy protocols for the treatment of orthotopic melanoma and glioblastoma tumors. These protocols utilize a small animal radiation research platform, which is an ideal radiation device for delivering localized and precise X-ray doses to the tumor mass. By employing these platforms, we aim to limit the side effects associated with irradiating healthy surrounding tissues. Our detailed protocols offer a valuable framework for conducting preclinical studies that closely mimic clinical radiotherapy techniques, bridging the gap between experimental results and patient outcomes. Competing Interests: Disclosures M.E.R.R. reports receiving research funding from Roche and Highlight Therapeutics. She also has received speaker's bureau honoraria from AZ, BMS and ROCHE. (Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training and similar technologies.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu