Using the DNA Integrity Number to Analyze DNA Quality in Specimens Collected from Liquid-Based Cytology after Fine-Needle Aspiration of Breast Tumors and Lesions.

Autor:	Hoshino A; Department of Diagnostic Pathology, Kitasato University Kitasato Institute Hospital, Tokyo, Japan, frog-hoshino@umin.ac.jp.; Department of Pathology, Kitasato University School of Medicine, Tokyo, Japan, frog-hoshino@umin.ac.jp., Oana Y; Department of Diagnostic Pathology, Kitasato University Kitasato Institute Hospital, Tokyo, Japan., Ohi Y; Department of Pathology, Sagara Hospital, Kagoshima City, Kagoshima, Japan., Maeda Y; Department of Pathology, Sagara Hospital, Kagoshima City, Kagoshima, Japan., Omori M; Department of Pathology, Kurashiki Medical Center, Kurashiki City, Okayama, Japan., Takada Y; Department of Pathology, Kurashiki Medical Center, Kurashiki City, Okayama, Japan., Ikeda T; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan., Sotome K; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan., Maeda H; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan., Yanagisawa T; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan., Takeuchi O; Biomedical Laboratory, Kitasato University Kitasato Institute Hospital, Tokyo, Japan., Kuronuma S; Biomedical Laboratory, Kitasato University Kitasato Institute Hospital, Tokyo, Japan., Sangai T; Department of Surgery, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan., Shibahara Y; Department of Pathology, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan., Murakumo Y; Department of Pathology, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan., Saegusa M; Department of Pathology, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan., Kanomata N; Department of Pathology, St. Lukes International Hospital, Tokyo, Japan., Nagasawa S; Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa City, Chiba, Japan., Yamaguchi R; Department of Pathology, Nagasaki University Hospital, Nagasaki City, Nagasaki, Japan., Yoshida M; Department of Diagnostic Pathology, National Cancer Center Hospital, Tokyo, Japan., Kozuka Y; Department of Pathology, Mie University Hospital, Tsu, Japan., Matsumoto H; Division of Breast Surgery, Saitama Cancer Center, Saitama, Japan., Tsugawa K; Divison of Breast and Endocrine Surgery, Department of Surgery, St. Marianna University School of Medicine, Kawasaki, Japan., Maeda I; Department of Diagnostic Pathology, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.; Department of Pathology, Kitasato University School of Medicine, Tokyo, Japan.
Jazyk:	angličtina
Zdroj:	Acta cytologica [Acta Cytol] 2024; Vol. 68 (2), pp. 145-152. Date of Electronic Publication: 2024 Mar 29.
DOI:	10.1159/000538071
Abstrakt:	Introduction: Cancer genome analysis using next-generation sequencing requires adequate and high-quality DNA samples. Genomic analyses were conventionally performed using formalin-fixed paraffin-embedded sections rather than cytology samples such as cell block or smear specimens. Specimens collected from liquid-based cytology (LBC) have the potential to be sources of high-quality DNA suitable for genetic analysis even after long-term storage. Methods: We collected breast tumor/lesion fractions from 92 residual LBC specimens using fine-needle aspiration (FNA) biopsy, including breast carcinoma (1 invasive carcinoma and 4 ductal carcinomas in situ), papillomatous lesion (5 intraductal papillomas), and fibroepithelial lesion (19 phyllodes tumors and 53 fibroadenomas) samples, and others (1 ductal adenoma, 1 hamartoma, 1 fibrocystic disease, and 7 unknown). DNA was extracted from all samples and subjected to DNA integrity number (DIN) score analysis. Results: Average DIN score collected from 92 LBC specimens was significantly higher score. In addition, high-quality DNA with high DIN values (7.39 ± 0.80) was successfully extracted more than 12 months after storage of residual LBC specimens. Conclusion: Residual LBC specimens collected from FNA of the breast were verified to carry high-quality DNA and could serve as an alternate source for genetic analysis. (© 2024 S. Karger AG, Basel.)
Databáze:	MEDLINE
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