Elucidating hepatoprotective potential of Cichorium intybus through multimodal assessment and molecular docking analysis with hepatic protective enzymes.

Autor: Pathak A; College of Veterinary & Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, US Nagar, Uttarakhand, India. Electronic address: drpathak1704@gmail.com., Singh SP; College of Veterinary & Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, US Nagar, Uttarakhand, India., Tiwari A; College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, US Nagar, Uttarakhand, India.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2024 May; Vol. 187, pp. 114595. Date of Electronic Publication: 2024 Mar 28.
DOI: 10.1016/j.fct.2024.114595
Abstrakt: This study employed a comprehensive approach to validate the hepatoprotective potential of phytoconstituents from Cichorium intybus leaves. In vitro, in vivo and in silico techniques were used to confirm the protective effects on liver enzymes. In vitro antioxidant assessment revealed the highest potential in the hydroethanolic leaf extract compared to aqueous and methanolic extracts. The study further investigated the ameliorative efficacy of the hydro-ethanolic extract (HECL) in male Wistar rats exposed to lead (50 mg/kg b wt.) and nickel (4.0 mg/kg b wt.) individually and in combination for 90 days. HECL at 250 mg/kg b wt. mitigated hepatic injury, oxidative stress, DNA fragmentation, ultrastructural and histopathological alterations induced by lead and nickel. Molecular docking explored the interaction of 28 phytoconstituents from C. intybus with hepatoprotective protein targets. Cyanidin and rutin exhibited the highest affinity for liver corrective enzymes among the screened phytoconstituents. These findings underscore the liver corrective potential of C. intybus leaf phytoconstituents, shedding light on their molecular interactions with hepatoprotective targets. This research contributes valuable insights into the therapeutic applications of C. intybus in liver protection.
Competing Interests: Declaration of competing interest All authors certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript entitled “Elucidating Hepatoprotective Potential of Cichorium intybus Through Multimodal Assessment and Molecular Docking Analysis with Hepatic Protective Enzymes”.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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