Insecticidal action of mammalian galectin-1-transfected Arabidopsis thaliana.

Autor: Liao ZH; Department of Life Science, College of Health Science and Technology, National Central University, Taoyuan, Taiwan., Shih HT; Applied Zoology Division, Taiwan Agricultural Research Institute, Ministry of Agriculture, Taichung, Taiwan, ROC., Dong YJ; Applied Zoology Division, Taiwan Agricultural Research Institute, Ministry of Agriculture, Taichung, Taiwan, ROC., Tseng MJ; Applied Zoology Division, Taiwan Agricultural Research Institute, Ministry of Agriculture, Taichung, Taiwan, ROC., Wang SH; Department of Life Science, College of Health Science and Technology, National Central University, Taoyuan, Taiwan., Chen SJ; Department of Life Science, Institute of Ecology and Evolutionary Biology and TechComm-5, College of Life Science, National Taiwan University, Taipei, Taiwan., Wu SJ; Department of Life Science, College of Health Science and Technology, National Central University, Taoyuan, Taiwan., Huang RN; Department of Entomology, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2024 Aug; Vol. 80 (8), pp. 4024-4033. Date of Electronic Publication: 2024 Apr 10.
DOI: 10.1002/ps.8109
Abstrakt: Background: Galectins (GALs) are a family of mammalian sugar-binding proteins specific for β-galactosides. Our previous studies have shown that the larval development of the diamondback moth (Plutella xylostella) is significantly disturbed when fed with recombinant mammalian galectin 1 (GAL1) derived from Escherichia coli. To further explore its applicability, two GAL1-overexpressed Arabidopsis [GAL1-Arabidopsis (whole plant) and GAL1-Arabidopsis-vas (vascular bundle-specific)] lines were established for insecticidal activity and mechanism studies.
Results: The expression level of GAL1 in transgenic Arabidopsis is 1-0.5% (GAL1-Arabidopsis) and 0.08-0.01% (GAL1-Arabidopsis-vas) of total leaf soluble protein. Survival, body weight, and food consumption significantly decreased in a time-dependent manner in P. xylostella larvae (with chewing mouthparts) fed on GAL1-Arabidopsis. The mortality of Kolla paulula (with piercing-sucking mouthparts and xylem feeder) fed on GAL1-Arabidopsis-vas was also significantly higher than that fed on wild-type Arabidopsis (WT-Arabidopsis), but was lower than that fed on GAL1-Arabidopsis. The histochemical structure and results of immunostaining suggested that the binding of GAL1 to the midgut epithelium of P. xylostella fed on GAL1-Arabidopsis was dose- and time-dependent. Ultrastructural studies further showed the disruption of microvilli, abnormalities in epithelial cells, and fragments of the peritrophic membrane (PM) in P. xylostella larvae fed on GAL1-Arabidopsis.
Conclusion: The insecticidal mechanism of GAL1 involves interference with PM integrity and suggests that GAL1 is a potential candidate for bioinsecticide development. © 2024 Society of Chemical Industry.
(© 2024 Society of Chemical Industry.)
Databáze: MEDLINE
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