Effect of chlorhexidine, povidone-iodine and betadine antiseptic eye drops on cultured human conjunctival goblet cell survival.

Autor: Hadad R; Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen E, Denmark., Hedengran A; Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen E, Denmark.; Department of Ophthalmology, Copenhagen University Hospital, Glostrup, Denmark., Barnils A; Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen E, Denmark., Petrovski G; Department of Ophthalmology, Center for eye Research and Innovative Diagnostics, Oslo University Hospital and Institute for Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.; Department of Ophthalmology, University of Split School of Medicine and University Hospital Centre, Split, Croatia., Cvenkel B; Department of Ophthalmology, University Medical Centre Ljubljana, Ljubljana, Slovenia.; Medical Faculty, University of Ljubljana, Ljubljana, Slovenia., Utheim TP; Department of Ophthalmology, Center for eye Research and Innovative Diagnostics, Oslo University Hospital and Institute for Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.; Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway., Dartt DA; Department of Ophthalmology, Harvard Medical School, Schepens Eye Research Institute, Massachusetts Eye and Ear, Boston, Massachusetts, USA., Heegaard S; Department of Ophthalmology, Copenhagen University Hospital, Glostrup, Denmark.; Department of Pathology, University Hospital Rigshospitalet, Copenhagen, Denmark., Kolko M; Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen E, Denmark.; Department of Ophthalmology, Copenhagen University Hospital, Glostrup, Denmark.
Jazyk: angličtina
Zdroj: Acta ophthalmologica [Acta Ophthalmol] 2024 Nov; Vol. 102 (7), pp. 773-778. Date of Electronic Publication: 2024 Mar 30.
DOI: 10.1111/aos.16684
Abstrakt: Purpose: To compare the effect of the ocular antiseptic treatments 0.05% chlorhexidine, 5% povidone-iodine (PI) and 5% betadine on cell viability and mucin secretion of primary cultured human goblet cells (GCs).
Method: GC viability was analysed using lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays. Expression of mucin was visualised by immunohistochemical MUC5AC staining.
Results: PI and betadine significantly reduced GC survival compared to the control (mean cell survival 23 ± 6% and 23 ± 7%, respectively, p < 0.05), whereas chlorhexidine did not significantly affect GC viability (mean cell survival: 78 ± 17%), as measured by the LDH assay. Similar results were obtained from the MTT assay, where PI and betadine caused a significant loss of GCs (mean cell survival: 26 ± 12% and 26 ± 13%, respectively, p < 0.05). Chlorhexidine did not significantly alter GC survival compared to the control (mean cell survival: 79 ± 8%). PI and betadine caused a dispersion of mucin secretion, which chlorhexidine did not.
Conclusion: The most used antiseptic treatments, PI and betadine, applied prior to ocular surgery are significantly more cytotoxic to conjunctival GCs than chlorhexidine treatment.
(© 2024 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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