Effect of leukoreduction on the omics phenotypes of canine packed red blood cells during refrigerated storage.

Autor: Miglio A; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia 06126, Italy., Rocconi F; Department of Veterinary Medicine, Veterinary University Hospital, University of Teramo, Località Piano D'Accio, Teramo 64100, Italy., Cremoni V; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia 06126, Italy., D'Alessandro A; Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, Colorado, USA., Reisz JA; Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, Colorado, USA., Maslanka M; Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, Colorado, USA., Lacroix IS; Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, Colorado, USA., Di Francesco D; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia 06126, Italy., Antognoni MT; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia 06126, Italy., Di Tommaso M; Department of Veterinary Medicine, Veterinary University Hospital, University of Teramo, Località Piano D'Accio, Teramo 64100, Italy.
Jazyk: angličtina
Zdroj: Journal of veterinary internal medicine [J Vet Intern Med] 2024 May-Jun; Vol. 38 (3), pp. 1498-1511. Date of Electronic Publication: 2024 Mar 29.
DOI: 10.1111/jvim.17031
Abstrakt: Background: Red blood cell (RBC) storage promotes biochemical and morphological alterations, collectively referred to as storage lesions (SLs). Studies in humans have identified leukoreduction (LR) as a critical processing step that mitigates SLs. To date no study has evaluated the impact of LR on metabolic SLs in canine blood units using omics technologies.
Objective: Compare the lipid and metabolic profiles of canine packed RBC (pRBC) units as a function of LR in fresh and stored refrigerated (up to 42 days) units.
Animals: Packed RBC units were obtained from 8 donor dogs enrolled at 2 different Italian veterinary blood banks.
Study Design and Methods: Observational study. A volume of 450 mL of whole blood was collected using Citrate-Phosphate-Dextrose-Saline-Adenine-Glucose-Mannitol (CPD-SAGM) transfusion bags with a LR filter to produce 2 pRBC units for each donor, without (nLR-pRBC) and with (LR-pRBC) LR. Units were stored in the blood bank at 4 ± 2°C. Sterile weekly samples were obtained from each unit for omics analyses.
Results: A significant effect of LR on fresh and stored RBC metabolic phenotypes was observed. The nLR-pRBC were characterized by higher concentrations of free short and medium-chain fatty acids, carboxylic acids (pyruvate, lactate), and amino acids (arginine, cystine). The LR-pRBC had higher concentrations of glycolytic metabolites, high energy phosphate compounds (adenosine triphosphate [ATP]), and antioxidant metabolites (pentose phosphate, total glutathione).
Conclusion and Clinical Importance: Leukoreduction decreases the metabolic SLs of canine pRBC by preserving energy metabolism and preventing oxidative lesions.
(© 2024 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.)
Databáze: MEDLINE
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