Phase transition of GvpU regulates gas vesicle clustering in bacteria.

Autor: Li Z; Department of Bioengineering, Rice University, Houston, TX, USA., Shen Q; Department of Bioengineering, Rice University, Houston, TX, USA., Usher ET; Department of Biomedical Engineering, Center for Biomolecular Condensates, Washington University in St. Louis, Saint Louis, MO, USA.; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO, USA., Anderson AP; Department of Bioengineering, Rice University, Houston, TX, USA., Iburg M; Department of Bioengineering, Rice University, Houston, TX, USA., Lin R; Department of Bioengineering, Rice University, Houston, TX, USA., Zimmer B; Department of Bioengineering, Rice University, Houston, TX, USA., Meyer MD; Shared Equipment Authority, Rice University, Houston, TX, USA., Holehouse AS; Department of Biomedical Engineering, Center for Biomolecular Condensates, Washington University in St. Louis, Saint Louis, MO, USA.; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO, USA., You L; Department of Biomedical Engineering, Duke University, Durham, NC, USA. you@duke.edu.; Center for Quantitative BioDesign, Duke University, Durham, NC, USA. you@duke.edu., Chilkoti A; Department of Biomedical Engineering, Duke University, Durham, NC, USA. chilkoti@duke.edu., Dai Y; Department of Biomedical Engineering, Center for Biomolecular Condensates, Washington University in St. Louis, Saint Louis, MO, USA. dyifan@wustl.edu.; Department of Biomedical Engineering, Duke University, Durham, NC, USA. dyifan@wustl.edu., Lu GJ; Department of Bioengineering, Rice University, Houston, TX, USA. george.lu@rice.edu.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2024 Apr; Vol. 9 (4), pp. 1021-1035. Date of Electronic Publication: 2024 Mar 29.
DOI: 10.1038/s41564-024-01648-3
Abstrakt: Gas vesicles (GVs) are microbial protein organelles that support cellular buoyancy. GV engineering has multiple applications, including reporter gene imaging, acoustic control and payload delivery. GVs often cluster into a honeycomb pattern to minimize occupancy of the cytosol. The underlying molecular mechanism and the influence on cellular physiology remain unknown. Using genetic, biochemical and imaging approaches, here we identify GvpU from Priestia megaterium as a protein that regulates GV clustering in vitro and upon expression in Escherichia coli. GvpU binds to the C-terminal tail of the core GV shell protein and undergoes a phase transition to form clusters in subsaturated solution. These properties of GvpU tune GV clustering and directly modulate bacterial fitness. GV variants can be designed with controllable sensitivity to GvpU-mediated clustering, enabling design of genetically tunable biosensors. Our findings elucidate the molecular mechanisms and functional roles of GV clustering, enabling its programmability for biomedical applications.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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