Application of the galactomannan gel from Cassia grandis seeds for biomedical purposes: Study of the incorporation of collagenases and their release profile.

Autor: de Albuquerque Lima Duarte C; Multiuser Biotechnology Laboratory of the Pernambuco Sertão, University of Pernambuco-UPE, Campus Arcoverde, Cícero Monteiro de Melo, 56503-146 Arcoverde, PE, Brazil; Biotechnology and Therapeutic Innovation Laboratory, University of Pernambuco-UPE, Campus Garanhuns, Rua Capitão Pedro Rodrigues, 55294-310 Garanhuns, PE, Brazil., da Silva MG; Biotechnology and Therapeutic Innovation Laboratory, University of Pernambuco-UPE, Campus Garanhuns, Rua Capitão Pedro Rodrigues, 55294-310 Garanhuns, PE, Brazil., Porto ALF; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco-UFRPE, Dom Manuel de Medeiros, 52171-900 Recife, PE, Brazil., de Albuquerque Wanderley MC; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco-UFRPE, Dom Manuel de Medeiros, 52171-900 Recife, PE, Brazil., da Silva SSS; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco-UFRPE, Dom Manuel de Medeiros, 52171-900 Recife, PE, Brazil., de Andrade AF; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco-UFRPE, Dom Manuel de Medeiros, 52171-900 Recife, PE, Brazil., Bezerra RP; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco-UFRPE, Dom Manuel de Medeiros, 52171-900 Recife, PE, Brazil., Converti A; Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, via Opera Pia 15, I-16145 Genoa, Italy., Ramos DG; Multiuser Biotechnology Laboratory of the Pernambuco Sertão, University of Pernambuco-UPE, Campus Arcoverde, Cícero Monteiro de Melo, 56503-146 Arcoverde, PE, Brazil; Integrated Laboratory of Applied Biotechnology, Biological Science Institute, University of Pernambuco (UPE), Rua Arnóbio Marques, 50100-130 Recife, PE, Brazil., de Araújo Viana Marques D; Integrated Laboratory of Applied Biotechnology, Biological Science Institute, University of Pernambuco (UPE), Rua Arnóbio Marques, 50100-130 Recife, PE, Brazil., de Albuquerque PBS; Biological Science Institute, University of Pernambuco (UPE), Rua Arnóbio Marques, 50100-130 Recife, PE, Brazil; Laboratory of Bioprospecting and Applied Ethnopharmacotoxicology (LABEA), University of Pernambuco-UPE, Campus Garanhuns, Rua Capitão Pedro Rodrigues, 55294-310 Garanhuns, PE, Brazil. Electronic address: priscilla.albuquerque@upe.br.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2024 Jun 15; Vol. 334, pp. 122061. Date of Electronic Publication: 2024 Mar 16.
DOI: 10.1016/j.carbpol.2024.122061
Abstrakt: The galactomannan-based gel from Cassia grandis seeds was used to incorporate Penicillium sp. UCP 1286 and commercial collagenases. Experiments were carried out according to a 2 3 -full factorial design to identify the most significant parameters for the incorporation process. The pH of the incorporation solution (pH i ), stirring time (t), and initial protein concentration in the crude extract (PC i ) were selected as the three independent variables, and the efficiency of collagenase incorporation (E) and collagenolytic activity (CA) after 360 min as the responses. pH i and PC i showed positive statistically significant effects on E, while CA was positively influenced by pH i and t, but negatively by PC i . The fungi collagenase was released from the gel following a pseudo-Fickian behavior. Additionally, no <76 % of collagenase was efficiently incorporated into the gel retaining a high CA (32.5-69.8 U/mL). The obtained results for the commercial collagenase (E = 93.88 %, CA = 65.8 U/mL, and n = 0.10) demonstrated a pseudo-Fickian behavior similar to the fungi-collagenase. The results confirm the biotechnological potential of the gel as an efficient matrix for the incorporation of catalytic compounds; additionally, the incorporation of collagenases was achieved by retaining the proteases CA and releasing them in a controlled manner.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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