Insulin signaling and mitochondrial phenotype of skeletal muscle are programmed in utero by maternal diabetes.

Autor: Klöppel E; Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate Course on Gynecology and Obtetrics, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, 18618-689, São Paulo State, Brazil; Laboratory of Translational Metabolism, Institute of Physiology (IPHYS) of the Czech Academy of Sciences (CAS), 142 00, Prague, Czech Republic., Cruz LL; Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate Course on Gynecology and Obtetrics, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, 18618-689, São Paulo State, Brazil; Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, 78600-000, Mato Grosso State, Brazil., Prado-Souza LFL; Center for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André, 09210-580, São Paulo State, Brazil., Eckhardt A; Laboratory of Translational Metabolism, Institute of Physiology (IPHYS) of the Czech Academy of Sciences (CAS), 142 00, Prague, Czech Republic., Corrente JE; Research Support Office, Botucatu Medical School, Sao Paulo State University (UNESP), 18618-689, São Paulo State, Brazil., Dos Santos DC; Department of Structural and Functional Biology, Institute of Biosciences, Sao Paulo State University (UNESP), 18618-689, São Paulo State, Brazil., Justulin LA; Department of Structural and Functional Biology, Institute of Biosciences, Sao Paulo State University (UNESP), 18618-689, São Paulo State, Brazil., Rodrigues T; Center for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André, 09210-580, São Paulo State, Brazil., Volpato GT; Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, 78600-000, Mato Grosso State, Brazil., Damasceno DC; Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate Course on Gynecology and Obtetrics, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, 18618-689, São Paulo State, Brazil. Electronic address: debora.damasceno@unesp.br.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2024 Jul 01; Vol. 588, pp. 112199. Date of Electronic Publication: 2024 Mar 27.
DOI: 10.1016/j.mce.2024.112199
Abstrakt: Maternal diabetes may influence glucose metabolism in adult offspring, an area with limited research on underlying mechanisms. Our study explored the impact of maternal hyperglycemia during pregnancy on insulin resistance development. Adult female Sprague-Dawley rats from control and diabetic mothers were mated, and their female offspring were monitored for 150 days. The rats were euthanized for blood and muscle samples. Maternal diabetes led to heightened insulin levels, increased HOMA-IR, elevated triglycerides, and a raised TyG index in adult offspring. Muscle samples showed a decreased protein expression of AMPK, PI3K, MAPK, DRP1, and MFF. These changes induced intergenerational metabolic programming in female pups, resulting in insulin resistance, dyslipidemia, and glucose intolerance by day 150. Findings highlight the offspring's adaptation to maternal hyperglycemia, involving insulin resistance, metabolic alterations, the downregulation of insulin signaling sensors, and disturbed mitochondrial morphology. Maintaining maternal glycemic control emerges as crucial in mitigating diabetes-associated disorders in adult offspring.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: