Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers.

Autor: Caspe SG; Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK; Instituto Nacional de Tecnología Agropecuaria, Av. Rivadavia 1439, C1033AAE, Buenos Aires, Argentina. Electronic address: gaston.caspe@moredun.ac.uk., Konrad JL; Consejo Nacional de Investigaciones Científicas y Técnicas, Blvd. 27 de Febrero 210 Bis, S2000EZP Rosario, Sante Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad del Nordeste, Juan Bautista Cabral 2131, W3402BKG, Corrientes, Argentina., Moore DP; Instituto Nacional de Tecnología Agropecuaria, Av. Rivadavia 1439, C1033AAE, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Blvd. 27 de Febrero 210 Bis, S2000EZP Rosario, Sante Fe, Argentina., Sala JM; Instituto Nacional de Tecnología Agropecuaria, Av. Rivadavia 1439, C1033AAE, Buenos Aires, Argentina., Della-Rosa P; Instituto Nacional de Tecnología Agropecuaria, Av. Rivadavia 1439, C1033AAE, Buenos Aires, Argentina., Ortega-Mora LM; Facultad de Ciencias Veterinarias, Universidad Complutense de Madrid, Av. Puerta de Hierro, s/n, Moncloa - Aravaca, 28040, Madrid, Spain., Bacigalupe DR; Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata, Avenue 7 No. 776, CP1900, La Plata, Buenos Aires, Argentina; Departamento de Zoonosis, Municipalidad de Rauch, Rivadavia 750, B7203 Rauch, Buenos Aires, Argentina., Venturini MC; Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata, Avenue 7 No. 776, CP1900, La Plata, Buenos Aires, Argentina., Campero CM; Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK., Barbeito CG; Consejo Nacional de Investigaciones Científicas y Técnicas, Blvd. 27 de Febrero 210 Bis, S2000EZP Rosario, Sante Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata, Avenue 7 No. 776, CP1900, La Plata, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Journal of comparative pathology [J Comp Pathol] 2024 Apr; Vol. 210, pp. 29-37. Date of Electronic Publication: 2024 Mar 28.
DOI: 10.1016/j.jcpa.2024.02.006
Abstrakt: Neospora caninum is an obligate intracellular parasite that causes abortion in ruminants. Different strains produce differences in the severity of disease outcomes. These differences may cause physiological or pathological changes in cells, modifying the intercellular interactions and intracellular transport pathways that could be evidenced by identifying the terminal sugars. This study aimed to characterize the oligosaccharide pattern in the bovine placenta and uterus after infection with tachyzoites of three different strains of N. caninum (Nc-1, Nc-6 Argentina and Nc Spain-7) during early gestation. Fourteen heifers were inoculated intravenously on day 70 of gestation with 2 × 10 8  N. caninum tachyzoites and samples of placentae and uteri were analysed by histology and lectin histochemistry. In the infected groups, severe placentitis was associated with changes in lectin binding in the vascular endothelium by Lens culinaris agglutinin (LCA), Pisum sativum agglutinin (PSA) and Ricinus communis I (RCA-I) lectins, in the epithelial cells of the endometrial glands by RCA-I, Dolichos biflorus agglutinin (DBA), succinylated wheat germ agglutinin, peanut agglutinin (PNA), concanavalin-A (CON-A), LCA, PSA and Phaseolus vulgaris erythroagglutinin (PHA-e), and in the trophoblast layer by PNA, CON-A, LCA, PSA, PHA-e, soybean agglutinin, RCA-I, DBA and Bandieraea simplicifolia agglutinin (BSA-I). The results suggest that N. caninum causes changes in the glycosylation pattern in the maternofetal interface tissues and might cause abortions in early gestation due to changes in the cellular structure of the placenta.
Competing Interests: Declaration of competing interests The authors declared no conflicts of interest in relation to the research, authorship or publication of this article. They also declared no financial or personal relationships with other people or organizations that could inappropriately influence or bias the content of the paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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