Secreted in Xylem 6 ( SIX6 ) Mediates Fusarium oxysporum f. sp. fragariae Race 1 Avirulence on FW1 -Resistant Strawberry Cultivars.

Autor:	Dilla-Ermita CJ; Crop Improvement and Protection Research, USDA-ARS, 1636 E. Alisal St., Salinas, CA 93905.; Department of Plant Sciences, University of California Davis, One Shields Ave., Davis, CA 95616., Goldman P; Crop Improvement and Protection Research, USDA-ARS, 1636 E. Alisal St., Salinas, CA 93905., Anchieta A; Crop Improvement and Protection Research, USDA-ARS, 1636 E. Alisal St., Salinas, CA 93905., Feldmann MJ; Department of Plant Sciences, University of California Davis, One Shields Ave., Davis, CA 95616., Pincot DDA; Department of Plant Sciences, University of California Davis, One Shields Ave., Davis, CA 95616., Famula RA; Department of Plant Sciences, University of California Davis, One Shields Ave., Davis, CA 95616., Vachev M; Department of Plant Sciences, University of California Davis, One Shields Ave., Davis, CA 95616., Cole GS; Department of Plant Sciences, University of California Davis, One Shields Ave., Davis, CA 95616., Knapp SJ; Department of Plant Sciences, University of California Davis, One Shields Ave., Davis, CA 95616., Klosterman SJ; Crop Improvement and Protection Research, USDA-ARS, 1636 E. Alisal St., Salinas, CA 93905., Henry PM; Crop Improvement and Protection Research, USDA-ARS, 1636 E. Alisal St., Salinas, CA 93905.
Jazyk:	angličtina
Zdroj:	Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2024 Jun; Vol. 37 (6), pp. 530-541. Date of Electronic Publication: 2024 Jun 24.
DOI:	10.1094/MPMI-02-24-0012-R
Abstrakt:	Fusarium oxysporum f. sp. fragariae ( Fof ) race 1 is avirulent on cultivars with the dominant resistance gene FW1 , while Fof race 2 is virulent on FW1 -resistant cultivars. We hypothesized there was a gene-for-gene interaction between a gene at the FW1 locus and an avirulence gene ( AvrFW1 ) in Fof race 1. To identify a candidate AvrFW1 , we compared genomes of 24 Fof race 1 and three Fof race 2 isolates. We found one candidate gene that was present in race 1, was absent in race 2, was highly expressed in planta, and was homologous to a known effector, secreted in xylem 6 ( SIX6 ). We knocked out SIX6 in two Fof race 1 isolates by homologous recombination. All SIX6 knockout transformants (Δ SIX6 ) gained virulence on FW1/fw1 cultivars, whereas ectopic transformants and the wildtype isolates remained avirulent. Δ SIX6 isolates were quantitatively less virulent on FW1/fw1 cultivars Fronteras and San Andreas than fw1/fw1 cultivars. Seedlings from an FW1/fw1 × fw1/fw1 population were genotyped for FW1 and tested for susceptibility to a SIX6 knockout isolate. Results suggested that additional minor-effect quantitative resistance genes could be present at the FW1 locus. This work demonstrates that SIX6 acts as an avirulence factor interacting with a resistance gene at the FW1 locus. The identification of AvrFW1 enables surveillance for Fof race 2 and provides insight into the mechanisms of FW1- mediated resistance. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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