Sex peptide receptor is not required for refractoriness to remating or induction of egg laying in Aedes aegypti.

Autor:	Amaro IA; Department of Entomology, Cornell University, Ithaca, NY 14853, USA., Wohl MP; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA., Pitcher S; Department of Entomology, Cornell University, Ithaca, NY 14853, USA., Alfonso-Parra C; Department of Entomology, Cornell University, Ithaca, NY 14853, USA., Avila FW; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Paige AS; Department of Biological Sciences, Columbia University, New York, NY 10027, USA., Helinski MEH; Department of Entomology, Cornell University, Ithaca, NY 14853, USA., Duvall LB; Department of Biological Sciences, Columbia University, New York, NY 10027, USA., Harrington LC; Department of Entomology, Cornell University, Ithaca, NY 14853, USA., Wolfner MF; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., McMeniman CJ; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Jazyk:	angličtina
Zdroj:	Genetics [Genetics] 2024 May 07; Vol. 227 (1).
DOI:	10.1093/genetics/iyae034
Abstrakt:	Across diverse insect taxa, the behavior and physiology of females dramatically changes after mating-processes largely triggered by the transfer of seminal proteins from their mates. In the vinegar fly Drosophila melanogaster, the seminal protein sex peptide (SP) decreases the likelihood of female flies remating and causes additional behavioral and physiological changes that promote fertility including increasing egg production. Although SP is only found in the Drosophila genus, its receptor, sex peptide receptor (SPR), is the widely conserved myoinhibitory peptide (MIP) receptor. To test the functional role of SPR in mediating postmating responses in a non-Drosophila dipteran, we generated 2 independent Spr-knockout alleles in the yellow fever mosquito, Aedes aegypti. Although SPR is needed for postmating responses in Drosophila and the cotton bollworm Helicoverpa armigera, Spr mutant Ae. aegypti show completely normal postmating decreases in remating propensity and increases in egg laying. In addition, injection of synthetic SP or accessory gland homogenate from D. melanogaster into virgin female mosquitoes did not elicit these postmating responses. Our results demonstrate that Spr is not required for these canonical postmating responses in Ae. aegypti, indicating that other, as yet unknown, signaling pathways are likely responsible for these behavioral switches in this disease vector. Competing Interests: Conflicts of interest: The author(s) declare no conflict of interest. (© The Author(s) 2024. Published by Oxford University Press on behalf of The Genetics Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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