Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island.

Autor: Mustika YR; Profession Program in Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.; Master Program in Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia., Kinasih KN; Profession Program in Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.; Master Program in Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia., Effendi MH; Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia., Puspitasari Y; Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia., Kurniawan SC; Master Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen, The Netherlands., Khairullah AR; Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia., Samodra MEE; Bachelor Program in Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia., Hasib A; School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD, Queensland., Agustin ALD; Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia., Moses IB; Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria., Silaen OSM; Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Senen, Jakarta, Indonesia.
Jazyk: angličtina
Zdroj: Open veterinary journal [Open Vet J] 2024 Feb; Vol. 14 (2), pp. 699-706. Date of Electronic Publication: 2024 Feb 29.
DOI: 10.5455/OVJ.2024.v14.i2.10
Abstrakt: Background: The discovery of antibiotic-resistant Enterobacteriaceae bacteria in wild animals is an indication of their potential for wildlife as a reservoir. Bats are natural reservoir hosts and a source of infection for several microorganisms and have the potential to become vectors for the spread of zoonotic diseases.
Aim: A study was conducted based on these characteristics to identify and detect the bla TEM gene in Eschericia coli isolated from bat excrements in Tanjung Ringgit Cave, East Lombok.
Methods: Bat fecal samples were firstly inoculated onto eosin methylene blue agar media. Recovered bacterial isolates were further characterized using standard microbiological techniques. Antimicrobial susceptibility testing was done using the Kirby-Bauer disc diffusion method. bla TEM gene detection was carried out using polymerase chain reaction (PCR).
Results: Out of the 150 bat fecal samples obtained from Tanjung Ringgit cave, Lombok Island, Indonesia, 56 (37%) were positive for E. coli . Eight (8) out of the 56 E. coli isolates that underwent antimicrobial susceptibility testing using the disc diffusion method were confirmed to be multidrug-resistant as they exhibited resistance to at least three different classes of antibiotics. Out of the eight (8) multidrug resistance E. coli isolates recovered from fecal samples of bats, 2 (two) harbored the bla TEM gene.
Conclusion: The discovery of the bla TEM gene in bat fecal samples indicates the potential for wild animals, especially bats, to spread ESBL resistance genes to the environment and to humans.
Competing Interests: The authors declare that there is no conflict of interest.
Databáze: MEDLINE
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