Targeted recovery of male cells in a male and female same-cell mixture.

Autor: Hogg J; Forensic & National Security Sciences Institute, Syracuse University, Syracuse, New York, USA., Vandepoele ACW; Forensic & National Security Sciences Institute, Syracuse University, Syracuse, New York, USA., Zaccheo N; Forensic & National Security Sciences Institute, Syracuse University, Syracuse, New York, USA., Schulte J; Institute of Forensic Medicine, University of Basel, Basel, Switzerland., Schulz I; Institute of Forensic Medicine, University of Basel, Basel, Switzerland., Dubois J; Acadiana Criminalistics Laboratory, New Iberia, Louisiana, USA., Frank M; Forensic & National Security Sciences Institute, Syracuse University, Syracuse, New York, USA., Marciano MA; Forensic & National Security Sciences Institute, Syracuse University, Syracuse, New York, USA.
Jazyk: angličtina
Zdroj: Journal of forensic sciences [J Forensic Sci] 2024 Jul; Vol. 69 (4), pp. 1183-1197. Date of Electronic Publication: 2024 Mar 29.
DOI: 10.1111/1556-4029.15514
Abstrakt: DNA mixture deconvolution in the forensic DNA community has been addressed in a variety of ways. "Front-end" methods that separate the cellular components of mixtures can provide a significant benefit over computational methods as there is no need to rely on models with inherent uncertainty to generate conclusions. Historically, cell separation methods have been investigated but have been largely ineffective due to high cost, unreliability, and the lack of proper instrumentation. However, the last decade has given rise to more innovative technology that can target and recover cells more effectively. This study focuses on the development and optimization of a method to selectively label and recover male cells in a mixture of male and female epithelial cells using a Y-chromosome labeling kit with DEPArray™ technology, whereby male cells are labeled and recovered into a single extraction-ready tube. Labeling efficiency was tested using freshly collected and aged buccal swabs where 70%-75% and 38% of male cells were labeled, respectively, with less than 1% false positives. DEPArray™ detection was assessed using single buccal epithelial cells where approximately 80% of labeled cells were identified as male. Mixtures (1:1, 1:10, male to female) yielded profiles that were predominantly single source male or those in which the male component was more easily interpreted. The male-specific labeling method was demonstrated to be both robust and reliable when used on freshly collected cells. While the DEPArray™ meditated detection and recovery had notable limitations, it still improved the interpretation of the male component in same-cell mixtures in more recently collected samples.
(© 2024 The Authors. Journal of Forensic Sciences published by Wiley Periodicals LLC on behalf of American Academy of Forensic Sciences.)
Databáze: MEDLINE
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