Mechanism of programmed cell death in the posterior silk gland of the silkworm, Bombyx mori, during pupation based on Ca 2+ homeostasis.

Autor: Gu H; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China., Zou H; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China., Cheng J; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China., Liu X; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China., Jiang Z; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China., Peng P; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China., Li F; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China.; Sericulture Institute of Soochow University, Soochow University, Suzhou, China., Li B; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China.; Sericulture Institute of Soochow University, Soochow University, Suzhou, China.
Jazyk: angličtina
Zdroj: Insect molecular biology [Insect Mol Biol] 2024 Dec; Vol. 33 (6), pp. 551-559. Date of Electronic Publication: 2024 Mar 28.
DOI: 10.1111/imb.12911
Abstrakt: The silkworm, Bombyx mori, is a complete metamorphosed economic insect, and the silk gland is a significant organ for silk protein synthesis and secretion. The silk gland completely degenerates during pupation, but the regulatory mechanism of programmed cell death (PCD) has not yet been understood. In the present study, we investigated the non-genetic pathway of 20E-induced PCD in the posterior silk gland (PSG) based on intracellular Ca 2+ levels. Silk gland morphology and silk gland index indicated rapid degeneration of silk gland during metamorphosis from mature silkworm (MS) to pupal day 1 (P1), and Ca 2+ levels within the PSG were found to peak during the pre-pupal day 1 (PP1) stage. Moreover, the results of autophagy and apoptosis levels within the PSG showed that autophagy was significantly increased in MS-PP1 periods, and significantly decreased in PP2 and P1 periods. Apoptosis was almost absent in MS-PP1 periods and significantly increased in PP2 and P1 periods. Additionally, western blotting results showed that autophagy preceded apoptosis, and the autophagy-promoting ATG5 was cleaved by calpain to the autophagy-inhibiting and apoptosis-promoting NtATG5 since PP1 period, while decreased autophagy was accompanied by increased apoptosis. Collectively, these findings suggest that Ca 2+ is a key factor in the shift from autophagy to apoptosis.
(© 2024 Royal Entomological Society.)
Databáze: MEDLINE
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