Multireceptor phage cocktail against Salmonella enterica to circumvent phage resistance.

Autor: Martinez-Soto CE; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Rd W, N1G 5C9, Guelph, Ontario, Canada.; Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, 50 Stone Rd E, N1G 2W1, Guelph, Ontario, Canada., McClelland M; Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, 811 Health Sciences Road, CA 92614, United States., Kropinski AM; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 419 Gordon St, Guelph, ON N1G 2W1, Canada., Lin JT; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Rd W, N1G 5C9, Guelph, Ontario, Canada., Khursigara CM; Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, 50 Stone Rd E, N1G 2W1, Guelph, Ontario, Canada., Anany H; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Rd W, N1G 5C9, Guelph, Ontario, Canada.; Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, 50 Stone Rd E, N1G 2W1, Guelph, Ontario, Canada.
Jazyk: angličtina
Zdroj: MicroLife [Microlife] 2024 Mar 21; Vol. 5, pp. uqae003. Date of Electronic Publication: 2024 Mar 21 (Print Publication: 2024).
DOI: 10.1093/femsml/uqae003
Abstrakt: Non-Typhoidal Salmonella (NTS) is one of the most common food-borne pathogens worldwide, with poultry products being the major vehicle for pathogenesis in humans. The use of bacteriophage (phage) cocktails has recently emerged as a novel approach to enhancing food safety. Here, a multireceptor Salmonella phage cocktail of five phages was developed and characterized. The cocktail targets four receptors: O-antigen, BtuB, OmpC, and rough Salmonella strains. Structural analysis indicated that all five phages belong to unique families or subfamilies. Genome analysis of four of the phages showed they were devoid of known virulence or antimicrobial resistance factors, indicating enhanced safety. The phage cocktail broad antimicrobial spectrum against Salmonella , significantly inhibiting the growth of all 66 strains from 20 serovars tested in vitro . The average bacteriophage insensitive mutant (BIM) frequency against the cocktail was 6.22 × 10 -6 in S . Enteritidis, significantly lower than that of each of the individual phages. The phage cocktail reduced the load of Salmonella in inoculated chicken skin by 3.5 log 10 CFU/cm 2 after 48 h at 25°C and 15°C, and 2.5 log 10 CFU/cm 2 at 4°C. A genome-wide transduction assay was used to investigate the transduction efficiency of the selected phage in the cocktail. Only one of the four phages tested could transduce the kanamycin resistance cassette at a low frequency comparable to that of phage P22. Overall, the results support the potential of cocktails of phage that each target different host receptors to achieve complementary infection and reduce the emergence of phage resistance during biocontrol applications.
Competing Interests: None declared.
(© His Majesty the King in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada, 2024.)
Databáze: MEDLINE
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