Chloroplast transformation in new cultivars of tomato through particle bombardment.

Autor: Tanwar N; TERI-Deakin Nano-Biotechnology Centre, The Energy Resources Institute (TERI), New Delhi, 110003 India.; School of Life and Environmental Sciences, Deakin University, Waurn Ponds Campus, Geelong, VIC 3216 Australia., Mahto BK; TERI-Deakin Nano-Biotechnology Centre, The Energy Resources Institute (TERI), New Delhi, 110003 India.; University Department of Botany, Ranchi University, Ranchi, Jharkhand 834008 India., Rookes JE; School of Life and Environmental Sciences, Deakin University, Waurn Ponds Campus, Geelong, VIC 3216 Australia., Cahill DM; School of Life and Environmental Sciences, Deakin University, Waurn Ponds Campus, Geelong, VIC 3216 Australia., Bansal KC; TERI-Deakin Nano-Biotechnology Centre, The Energy Resources Institute (TERI), New Delhi, 110003 India.; National Academy of Agricultural Sciences, New Delhi, 110012 India., Lenka SK; TERI-Deakin Nano-Biotechnology Centre, The Energy Resources Institute (TERI), New Delhi, 110003 India.; Present Address: Department of Plant Biotechnology, Gujarat Biotechnology University, Gujarat International Finance Tec (GIFT)-City, North Gate, Gandhinagar, 382355 Gujarat India.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2024 Apr; Vol. 14 (4), pp. 120. Date of Electronic Publication: 2024 Mar 25.
DOI: 10.1007/s13205-024-03954-3
Abstrakt: A protocol has been established for genetic transformation of the chloroplasts in two new cultivars of tomato ( Solanum lycopersicum  L.) grown in India and Australia: Pusa Ruby and Yellow Currant. Tomato cv. Green Pineapple was also used as a control that has previously been used for establishing chloroplast transformation by other researchers. Selected tomato cultivars were finalized from ten other tested cultivars (Green Pineapple excluded) due to their high regeneration potential and better response to chloroplast transformation. This protocol was set up using a chloroplast transformation vector ( pRB94 ) for tomatoes that is made up of a synthetic gene operon. The vector has a chimeric aadA selectable marker gene that is controlled by the rRNA operon promoter ( Prrn ). This makes the plant or chloroplasts resistant to spectinomycin and streptomycin. After plasmid-coated particle bombardment, leaf explants were cultured in 50 mg/L selection media. Positive explant selection from among all the dead-appearing (yellow to brown) explants was found to be the major hurdle in the study. Even though this study was able to find plastid transformants in heteroplasmic conditions, it also found important parameters and changes that could speed up the process of chloroplast transformation in tomatoes, resulting in homoplasmic plastid-transformed plants.
Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-024-03954-3.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest in the publication.
(© King Abdulaziz City for Science and Technology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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