Towards genomic-Newborn Screening: Technical feasibility of Exome Sequencing starting from dried blood spots.

Autor:	Mauri A; Pediatric Clinical Research Center 'Romeo ed Enrica Invernizzi', Department of Biomedical and Clinical Sciences, University of Milano, 20157 Milano, Italy.; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Berardo C; Pediatric Clinical Research Center 'Romeo ed Enrica Invernizzi', Department of Biomedical and Clinical Sciences, University of Milano, 20157 Milano, Italy.; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Biganzoli D; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Meta A; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Benedetti S; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Rey F; Pediatric Clinical Research Center 'Romeo ed Enrica Invernizzi', Department of Biomedical and Clinical Sciences, University of Milano, 20157 Milano, Italy.; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Messa L; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy.; Department of Electronics, Information and Bioengineering (DEIB), Politecnico di Milano, 20133 Milano, Italy., Zuccotti GV; Pediatric Clinical Research Center 'Romeo ed Enrica Invernizzi', Department of Biomedical and Clinical Sciences, University of Milano, 20157 Milano, Italy.; Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Carelli S; Pediatric Clinical Research Center 'Romeo ed Enrica Invernizzi', Department of Biomedical and Clinical Sciences, University of Milano, 20157 Milano, Italy.; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Alberti L; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy., Cereda C; Center of Functional Genomics and Rare diseases, Department of Pediatrics, Buzzi Children's Hospital, 20154 Milano, Italy.
Jazyk:	angličtina
Zdroj:	Molecular genetics and metabolism reports [Mol Genet Metab Rep] 2024 Mar 20; Vol. 39, pp. 101074. Date of Electronic Publication: 2024 Mar 20 (Print Publication: 2024).
DOI:	10.1016/j.ymgmr.2024.101074
Abstrakt:	Each year thousands of babies are born with rare genetic disorders not identified by current NBS panels, due to programs which are not yet optimal. Next-generation sequencing technologies have the potential to overcome many NBS drawbacks and provide large amounts of molecular data, broadening the number of diseases investigated. Here, we design and set up an NGS-based approach to evaluate the feasibility of NGS from dried blood spot starting from 34 DBSs. After assessing gDNA yield and integrity, libraries were performed using three target enrichment approaches, sequenced on NS500 platform, and analyzed on commercial platform. Specifically, we focus on virtual gene panels related to highly actionable neonatal/pediatric disorders. WES show that amount and quality of DBS-extracted gDNA are suitable for high-throughput sequencing. We obtain 500-1500 ng for each specimen, 1.7-1.8 260/280 wavelength, and DIN of 7 resulting DNA integrity, on par with traditional venous blood collection. A high read depth with 94.3% coverage uniformity is achieved for all samples. Data results on mean coverage are comparable among the different workflows tested and demonstrate that DBS from newborn collected at birth is a suitable material for the developing of gNBS programs. Competing Interests: The authors declare no conflicts of interest. (© 2024 The Authors.)
Databáze:	MEDLINE
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