Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples.

Autor:	Farkas K; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Kevill JL; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Williams RC; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Pântea I; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Ridding N; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Lambert-Slosarska K; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Woodhall N; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Grimsley JMS; UK Health Security Agency, Data Analytics & Surveillance Group, 10 South Colonnade, Canary Wharf, London E14 4PU, United Kingdom.; The London Data Company, London EC2N 2AT, United Kingdom., Wade MJ; UK Health Security Agency, Data Analytics & Surveillance Group, 10 South Colonnade, Canary Wharf, London E14 4PU, United Kingdom., Singer AC; UK Centre for Ecology & Hydrology, Wallingford OX10 8BB, United Kingdom., Weightman AJ; Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3AX, United Kingdom., Cross G; Science Evidence Advice Division, Health and Social Services Group, Welsh Government, Cathays Park, Cardiff CF10 3NQ, United Kingdom., Jones DL; School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom.; Food Futures Institute, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
Jazyk:	angličtina
Zdroj:	FEMS microbes [FEMS Microbes] 2024 Mar 05; Vol. 5, pp. xtae007. Date of Electronic Publication: 2024 Mar 05 (Print Publication: 2024).
DOI:	10.1093/femsmc/xtae007
Abstrakt:	Wastewater-based epidemiology is now widely used in many countries for the routine monitoring of SARS-CoV-2 and other viruses at a community level. However, efficient sample processing technologies are still under investigation. In this study, we compared the performance of the novel Nanotrap® Microbiome Particles (NMP) concentration method to the commonly used polyethylene glycol (PEG) precipitation method for concentrating viruses from wastewater and their subsequent quantification and sequencing. For this, we first spiked wastewater with SARS-CoV-2, influenza and measles viruses and norovirus and found that the NMP method recovered 0.4%-21% of them depending on virus type, providing consistent and reproducible results. Using the NMP and PEG methods, we monitored SARS-CoV-2, influenza A and B viruses, RSV, enteroviruses and norovirus GI and GII and crAssphage in wastewater using quantitative PCR (qPCR)-based methods and next-generation sequencing. Good viral recoveries were observed for highly abundant viruses using both methods; however, PEG precipitation was more successful in the recovery of low-abundance viruses present in wastewater. Furthermore, samples processed with PEG precipitation were more successfully sequenced for SARS-CoV-2 than those processed with the NMP method. Virus recoveries were enhanced by high sample volumes when PEG precipitation was applied. Overall, our results suggest that the NMP concentration method is a rapid and easy virus concentration method for viral targets that are abundant in wastewater, whereas PEG precipitation may be more suited to the recovery and analysis of low-abundance viruses and for next generation sequencing. Competing Interests: The authors have no relevant financial or non-financial interests to disclose. (© The Author(s) 2024. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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