HMGB2 Promotes De Novo Lipogenesis to Accelerate Hepatocyte Proliferation During Liver Regeneration.

Autor:	Choijookhuu N; Department of Anatomy, Histochemistry and Cell Biology.; Faculty of Medicine, University of Miyazaki, Miyazaki, Japan; and Department of Pathology and Forensic Medicine, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia., Yano K; Department of Anatomy, Histochemistry and Cell Biology.; Department of Surgery., Lkham-Erdene B; Department of Anatomy, Histochemistry and Cell Biology., Shirouzu S; Department of Anatomy, Histochemistry and Cell Biology.; Department of Oral and Maxillofacial Surgery., Kubota T; Department of Anatomy, Histochemistry and Cell Biology.; Department of Oral and Maxillofacial Surgery., Fidya; Department of Anatomy, Histochemistry and Cell Biology., Ishizuka T; Department of Anatomy, Histochemistry and Cell Biology., Kai K; Department of Anatomy, Histochemistry and Cell Biology.; Department of Surgery., Chosa E; Department of Orthopaedic Surgery., Hishikawa Y; Department of Anatomy, Histochemistry and Cell Biology.
Jazyk:	angličtina
Zdroj:	The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society [J Histochem Cytochem] 2024 Apr; Vol. 72 (4), pp. 245-264. Date of Electronic Publication: 2024 Mar 27.
DOI:	10.1369/00221554241241569
Abstrakt:	Liver regeneration is a well-orchestrated compensatory process that is regulated by multiple factors. We recently reported the importance of the chromatin protein, a high-mobility group box 2 (HMGB2) in mouse liver regeneration. However, the molecular mechanism remains unclear. In this study, we aimed to study how HMGB2 regulates hepatocyte proliferation during liver regeneration. Seventy-percent partial hepatectomy (PHx) was performed in wild-type (WT) and HMGB2-knockout (KO) mice, and the liver tissues were used for microarray, immunohistochemistry, quantitative polymerase chain reaction (qPCR), and Western blotting analyses. In the WT mice, HMGB2-positive hepatocytes colocalized with cell proliferation markers. In the HMGB2-KO mice, hepatocyte proliferation was significantly decreased. Oil Red O staining revealed the transient accumulation of lipid droplets at 12-24 hr after PHx in the WT mouse livers. In contrast, decreased amount of lipid droplets were found in HMGB2-KO mouse livers, and it was preserved until 36 hr. The microarray, immunohistochemistry, and qPCR results demonstrated that the expression of lipid metabolism-related genes was significantly decreased in the HMGB2-KO mouse livers. The in vitro experiments demonstrated that a decrease in the amount of lipid droplets correlated with decreased cell proliferation activity in HMGB2-knockdown cells. HMGB2 promotes de novo lipogenesis to accelerate hepatocyte proliferation during liver regeneration. Competing Interests: Competing InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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