| Autor: |
Granovskiy DL; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Khudainazarova NS; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Evtushenko EA; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Ryabchevskaya EM; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Kondakova OA; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Arkhipenko MV; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Kovrizhko MV; Rostov Research Institute of Microbiology and Parasitology, 344010 Rostov-On-Don, Russia., Kolpakova EP; Rostov Research Institute of Microbiology and Parasitology, 344010 Rostov-On-Don, Russia., Tverdokhlebova TI; Rostov Research Institute of Microbiology and Parasitology, 344010 Rostov-On-Don, Russia., Nikitin NA; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Karpova OV; Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia. |
| Abstrakt: |
Rotavirus infection is a leading cause of severe dehydrating gastroenteritis in children under 5 years of age. Although rotavirus-associated mortality has decreased considerably because of the introduction of the worldwide rotavirus vaccination, the global burden of rotavirus-associated gastroenteritis remains high. Current vaccines have a number of disadvantages; therefore, there is a need for innovative approaches in rotavirus vaccine development. In the current study, a universal recombinant rotavirus antigen (URRA) for a novel recombinant vaccine candidate against rotavirus A was obtained and characterised. This antigen included sequences of the VP8* subunit of rotavirus spike protein VP4. For the URRA, for the first time, two approaches were implemented simultaneously-the application of a highly conserved neutralising epitope and the use of the consensus of the extended protein's fragment. The recognition of URRA by antisera to patient-derived field rotavirus isolates was proven. Plant virus-based spherical particles (SPs), a novel, effective and safe adjuvant, considerably enhanced the immunogenicity of the URRA in a mouse model. Given these facts, a URRA + SPs vaccine candidate is regarded as a prospective basis for a universal vaccine against rotavirus. |
