Presence of Zonula Occludens Toxin-Coding Genes among Vibrio parahaemolyticus Isolates of Clinical and Environmental Origin.

Autor:	Iribarren C; Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8320000, Chile., Plaza N; Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8320000, Chile., Ramírez-Araya S; Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8320000, Chile., Pérez-Reytor D; Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8320000, Chile., Urrutia ÍM; Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8320000, Chile., Suffredini E; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, 00161 Rome, Italy., Vicenza T; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, 00161 Rome, Italy., Ulloa S; Subdepartment of Genomic and Molecular Genetics, Public Health Institute of Chile, Santiago 7780050, Chile., Fernández J; Subdepartment of Genomic and Molecular Genetics, Public Health Institute of Chile, Santiago 7780050, Chile., Navarrete P; Microbiology and Probiotics Laboratory, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago 7830490, Chile., Jaña V; Núcleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronómicas (NIAVA), Universidad de Las Américas, Santiago 7500975, Chile., Pavez L; Núcleo de Investigaciones en Ciencias Biológicas, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Santiago 7500000, Chile., Del Pozo T; Núcleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronómicas (NIAVA), Universidad de Las Américas, Santiago 7500975, Chile., Corsini G; Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8320000, Chile., Lopez-Joven C; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile., García K; Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8320000, Chile.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2024 Feb 29; Vol. 12 (3). Date of Electronic Publication: 2024 Feb 29.
DOI:	10.3390/microorganisms12030504
Abstrakt:	In recent studies, emphasis has been placed on the zonula occludens toxin (Zot) from the non-toxigenic Vibrio parahaemolyticus strain PMC53.7 as an agent inducing alterations in the actin cytoskeleton of infected Caco-2 cells and which appears as a relevant virulence factor. Universal zot primers were designed by the alignment of different types of zot gene and identification of conserved sequences to investigate the presence in diverse environmental and clinical V. parahaemolyticus isolates, in co-occurrence with virulence factors, such a hemolysins and secretion systems. The study screened a total of 390 isolates from environmental sources from Chile and Italy and 95 Chilean clinical isolates. The results revealed that around 37.2% of Chilean environmental strains and 25.9% of Italian strains, and 24.2% of clinical isolates carried the zot gene. The Zot-C2 cluster was present in 71.4% of Chilean environmental strains but absent in clinical isolates, while the Zot-C4 cluster was identified in 28.6% of environmental and 100% of clinical isolates. Understanding the role of zot in V. parahaemolyticus virulence is crucial, especially considering the risk associated with consuming diverse isolates from bivalves and the co-occurrence with virulence factors such as TDH, TRH or T3SS2.
Databáze:	MEDLINE
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