| Autor: |
Djehiche C; Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif 1, Setif 19000, Algeria., Benzidane N; Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif 1, Setif 19000, Algeria., Djeghim H; Biochemistry Laboratory, Division of Biotechnology and Health, Biotechnology Research Center (CRBt), Constantine 25000, Algeria., Tebboub M; Department of Mechanical Engineering, Faculty of Science and Technology, University Mentouri Brothers Constantine 1, Constantine 25000, Algeria., Mokrani EH; Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences, University Mentouri Brothers Constantine 1, Constantine 25000, Algeria., Mebrek S; Biochemistry Laboratory, Division of Biotechnology and Health, Biotechnology Research Center (CRBt), Constantine 25000, Algeria., Messaoudi M; Nuclear Research Centre of Birine, P.O. Box 180, Ain Oussera 17200, Algeria., Bensouici C; Biochemistry Laboratory, Division of Biotechnology and Health, Biotechnology Research Center (CRBt), Constantine 25000, Algeria., Alsalme A; Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Cornu D; Institut Européen des Membranes (IEM), UMR 5635, Univ. Montpellier, ENSCM, CNRS, Place Eugène Bataillon, 34095 Montpellier, France., Bechelany M; Institut Européen des Membranes (IEM), UMR 5635, Univ. Montpellier, ENSCM, CNRS, Place Eugène Bataillon, 34095 Montpellier, France.; Functional Materials Group, Gulf University for Science and Technology (GUST), Mubarak Al-Abdullah 32093, Kuwait., Arrar L; Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif 1, Setif 19000, Algeria., Barhoum A; NanoStruc Research Group, Chemistry Department, Faculty of Science, Helwan University, Cairo 11795, Egypt. |
| Abstrakt: |
Ammodaucus leucotrichus exhibits promising pharmacological activity, hinting at anti-inflammatory and anti-arthritic effects. This study investigated seed extracts from Ammodaucus leucotrichus using methanol and n-hexane, focusing on anti-inflammatory and anti-arthritic properties. The methanol extract outperformed the n-hexane extract and diclofenac, a reference anti-inflammatory drug, in trypsin inhibition (85% vs. 30% and 64.67% at 125 μg/mL). For trypsin inhibition, the IC50 values were 82.97 μg/mL (methanol), 202.70 μg/mL (n-hexane), and 97.04 μg/mL (diclofenac). Additionally, the n-hexane extract surpassed the methanol extract and diclofenac in BSA (bovine serum albumin) denaturation inhibition (90.4% vs. 22.0% and 51.4% at 62.5 μg/mL). The BSA denaturation IC50 values were 14.30 μg/mL (n-hexane), 5408 μg/mL (methanol), and 42.30 μg/mL (diclofenac). Gas chromatography-mass spectrometry (GC-MS) revealed 59 and 58 secondary metabolites in the methanol and n-hexane extracts, respectively. The higher therapeutic activity of the methanol extract was attributed to hydroxyacetic acid hydrazide, absent in the n-hexane extract. In silico docking studies identified 28 compounds with negative binding energies, indicating potential trypsin inhibition. The 2-hydroxyacetohydrazide displayed superior inhibitory effects compared to diclofenac. Further mechanistic studies are crucial to validate 2-hydroxyacetohydrazide as a potential drug candidate for rheumatoid arthritis treatment. |
