| Autor: |
Lee EB; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Chakravarthi VP; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Mohamadi R; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Dahiya V; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Vo K; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Ratri A; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Fields PE; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Marsh CA; Department of Obstetrics and Gynecology, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA., Rumi MAK; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center (KUMC), Kansas City, KS 66160, USA. |
| Abstrakt: |
Loss of ERβ increases primordial follicle growth activation (PFGA), leading to premature ovarian follicle reserve depletion. We determined the expression and gene regulatory functions of ERβ in dormant primordial follicles (PdFs) and activated primary follicles (PrFs) using mouse models. PdFs and PrFs were isolated from 3-week-old Erβ knockout ( Erβ null ) mouse ovaries, and their transcriptomes were compared with those of control Erβ fl/fl mice. We observed a significant (≥2-fold change; FDR p -value ≤ 0.05) deregulation of approximately 5% of genes (866 out of 16,940 genes, TPM ≥ 5) in Erβ null PdFs; ~60% (521 out of 866) of the differentially expressed genes (DEGs) were upregulated, and 40% were downregulated, indicating that ERβ has both transcriptional enhancing as well as repressing roles in dormant PdFs. Such deregulation of genes may make the Erβ null PdFs more susceptible to increased PFGA. When the PdFs undergo PFGA and form PrFs, many new genes are activated. During PFGA of Erβ fl/fl follicles, we detected a differential expression of ~24% genes (4909 out of 20,743; ≥2-fold change; FDR p -value ≤ 0.05; TPM ≥ 5); 56% upregulated and 44% downregulated, indicating the gene enhancing and repressing roles of Erβ-activated PrFs. In contrast, we detected a differential expression of only 824 genes in Erβ null follicles during PFGA (≥2-fold change; FDR p -value ≤ 0.05; TPM ≥ 5). Moreover, most (~93%; 770 out of 824) of these DEGs in activated Erβ null PrFs were downregulated. Such deregulation of genes in Erβ null activated follicles may impair their inhibitory role on PFGA. Notably, in both Erβ null PdFs and PrFs, we detected a significant number of epigenetic regulators and transcription factors to be differentially expressed, which suggests that lack of ERβ either directly or indirectly deregulates the gene expression in PdFs and PrFs, leading to increased PFGA. |
