| Autor: |
Su W; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310023, China., Mastova AV; Institute of Chemical Kinetics and Combustion, 630090 Novosibirsk, Russia., Ul'yanova MA; Institute of Chemical Kinetics and Combustion, 630090 Novosibirsk, Russia., Kononova PA; Institute of Chemical Kinetics and Combustion, 630090 Novosibirsk, Russia., Selyutina OY; Institute of Chemical Kinetics and Combustion, 630090 Novosibirsk, Russia.; Institute of Solid State Chemistry and Mechanochemistry, 630128 Novosibirsk, Russia., Evseenko VI; Institute of Solid State Chemistry and Mechanochemistry, 630128 Novosibirsk, Russia., Meteleva ES; Institute of Solid State Chemistry and Mechanochemistry, 630128 Novosibirsk, Russia., Dushkin AV; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310023, China.; Institute of Solid State Chemistry and Mechanochemistry, 630128 Novosibirsk, Russia., Su W; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310023, China., Polyakov NE; Institute of Chemical Kinetics and Combustion, 630090 Novosibirsk, Russia.; Institute of Solid State Chemistry and Mechanochemistry, 630128 Novosibirsk, Russia. |
| Abstrakt: |
Crocin is a unique water-soluble carotenoid found in crocus and gardenia flowers. Crocin has been shown to have a variety of pharmacological activities, such as antioxidant, anti-cancer, memory improvement, antidepressant, anti-ischemia, blood pressure lowering and aphrodisiac, gene protection and detoxification activities. Due to their amphiphilicity, crocin molecules form concentration-dependent self-associates (micelles) in a water solution. In the present study, using various NMR techniques (T 2 relaxation and selective gradient NOESY), we have demonstrated that crocin forms mixed micelles with water-soluble drug delivery system glycyrrhizin and linoleic acid molecules. Note, that the spin-spin T 2 relaxation time and NOESY spectroscopy are very sensitive to intermolecular interactions and molecular diffusion mobility. The second purpose of this work was the elucidation of the interaction of crocin with a model lipid membrane using NMR techniques and a molecular dynamics simulation and its effects on lipid oxidation. It was shown that the crocin molecule is located near the surface of the lipid bilayer and effectively protects lipids from oxidation by peroxyl radicals. The role of glycyrrhizin and vitamin C in metal-induced lipid oxidation was also elucidated. The results of this study may be useful for expanding the field of application of crocin in medicine and in the food industry. |
