| Autor: |
Jiménez-Trejo F; Laboratory of Cellular and Tissular Morphology, National Institute of Pediatrics, Insurgentes Sur No. 3700-C. Coyoacán, Mexico City 04530, Mexico., Arriaga-Canon C; National Institute of Cancerology, Av. San Fernando No. 22, Tlalpan, Section XVI, Mexico City 14080, Mexico., Herrera LA; Biomedical Research Unit in Cancer, National Institute of Cancerology-Institute of Biomedical Research, UNAM, Tlalpan, Mexico City 14080, Mexico.; Tecnológico de Monterrey, School of Medicine and Health Sciences, Monterrey 64710, Mexico., Coronado-Mares I; Tlanepantla Regional Hospital, Av. Paseo del Ferrocarril No. 88, Tlanepantla de Baz 54090, Mexico., Montiel-Manríquez R; National Institute of Cancerology, Av. San Fernando No. 22, Tlalpan, Section XVI, Mexico City 14080, Mexico., González-Santoyo I; Faculty of Psychology, Universidad Nacional Autónoma de México, Circuito Escolar S/N, Ciudad Universitaria, Mexico City 04510, Mexico., Pérez-Báez WB; National Institute of Cancerology, Av. San Fernando No. 22, Tlalpan, Section XVI, Mexico City 14080, Mexico., Tapia-Rodríguez M; Microscopy Core Facility, Biomedical Research Institute, Universidad Nacional Autónoma de México, Tercer Circuito Exterior S/N, Ciudad Universitaria, Mexico City 04510, Mexico. |
| Abstrakt: |
Cryptorchidism (CO) or undescended testes is defined as the failure of one or both testes to be positioned inside the scrotum. Typically, cryptorchidism is detected at birth or shortly thereafter, and in humans, it is considered to be part of the testicular dysgenesis syndrome (TDS), a complex pathology regarding the male reproductive system that apparently involves the interaction of both genetic and environmental harmful factors, mainly during embryonic development. Serotonin (5-HT) is an ancient molecule that participates in a broad range of body functions, and in recent years, its importance in reproduction has started to be elucidated. In male pathologies such as infertility, varicocele, erectile dysfunction, and primary carcinoid tumors, an increase in 5-HT concentration or its metabolites in the blood, semen, and urine has been directly related; nevertheless, the role of 5-HT in CO remains unknown. In the present work, our goal was to answer two important questions: (1) whether some serotonergic system components are present in adult male Oryctolagus cuniculus (chinchilla rabbit) and (2) if there are changes in their expression in an experimental model of CO. Using histological, molecular, and biochemical approaches, we found the presence of some serotonergic system components in the adult chinchilla rabbit, and we demonstrated that its expression is downregulated after CO was pharmacologically induced. Although we did not test the role of 5-HT in the etiology of CO, our results suggest that this indoleamine could be important for the regulation of steroidogenesis and spermatogenesis processes in the chinchilla rabbit during adulthood. Finally, in parallel experimental series, we found downregulation of kynurenine concentration in COI rabbits when compared to control ones, suggesting that CO could be affecting the kynurenine pathway and probably testicular immune privilege which in turn could lead to infertility/sterility conditions in this disorder. |
