| Autor: |
Sadiq S; Combined Military Hospital, Khariyan Medical College, Gujrat 49600, Pakistan., Anwar MZ; Combined Military Hospital, Khariyan Medical College, Gujrat 49600, Pakistan., Shafique H; Institute of Cellular Medicine, Newcastle University Medical School, Newcastle University, Newcastle upon Tyne NE1 7RU, UK., Manzoor SM; Combined Military Hospital, Khariyan Medical College, Gujrat 49600, Pakistan., Shoaib S; Combined Military Hospital, Khariyan Medical College, Gujrat 49600, Pakistan., Hamid R; Combined Military Hospital, Khariyan Medical College, Gujrat 49600, Pakistan., Hashmi SN; Combined Military Hospital, Khariyan Medical College, Gujrat 49600, Pakistan., Ashraf NM; School of Biochemistry & Biotechnology, University of the Punjab, Lahore 54590, Pakistan., Afsar T; Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia., Bhat MA; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Razak S; Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia. |
| Abstrakt: |
Background and Objectives : A polymorphism in the promoter region of the IL-6 gene would influence the level of IL-6 expression in patients with HCV, resulting in a pro-inflammatory response. Few studies have shown the association between -174G>C (rs1800795) and -1363G>T (rs2069827) polymorphisms and HCV infection, and their results have been contradictory. There are no data published in our population to study such an IL-6 stimulus against HCV infection and its impact on RNA secondary structure. Therefore, we isolated human subjects from the province of Punjab, Pakistan. The objective was to screen for IL-6 gene promoter polymorphisms -174G/C and -1363G/T and those correlated with serum concentrations of IL-6 in patients with HCV and compared with a control. Materials and Methods : In conventional PCR, measurement of serum IL-6 by CLIA and statistical analysis were performed to observe the genotype association studies. By integrating bioinformatics and computational tools, our study aimed to provide a comprehensive understanding of how variations in the promoter region of IL-6 may have functional implications on gene expression. Results : The -174G>C and -1363G>T genotypes in the promoter region of patients with HCV were in strong allelic association (Δ = 0.97, p < 0.001). Interestingly, the bioinformatics analysis was well aligned with our experimental data. Conclusions : Based on the data, it can be inferred that IL-6 gene promoter polymorphisms are important in the dysregulation of IL-6 levels in patients with HCV. |
