| Autor: |
Rendek T; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, 811 08 Bratislava, Slovakia., Saade R; 2nd Department of Gynaecology and Obstetrics, Faculty of Medicine, Comenius University, 811 08 Bratislava, Slovakia., Pos O; Geneton Ltd., 841 04 Bratislava, Slovakia.; Science Park, Comenius University, 841 04 Bratislava, Slovakia., Kolnikova G; Department of Pathological Anatomy, National Cancer Institute, 833 10 Bratislava, Slovakia., Urbanova M; Department of Pathological Anatomy, National Cancer Institute, 833 10 Bratislava, Slovakia., Budis J; Geneton Ltd., 841 04 Bratislava, Slovakia.; Science Park, Comenius University, 841 04 Bratislava, Slovakia., Mihok L; Department of Medical Genetics, National Cancer Institute, 833 10 Bratislava, Slovakia., Tomas M; National Cancer Institute, Surgical Oncology Clinic of Slovak Medical University, 833 10 Bratislava, Slovakia., Szemes T; Geneton Ltd., 841 04 Bratislava, Slovakia.; Science Park, Comenius University, 841 04 Bratislava, Slovakia., Repiska V; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, 811 08 Bratislava, Slovakia. |
| Abstrakt: |
Slovakia has one of the highest rates of colorectal cancer among the developed countries, ranking as the second highest in the incidence of this disease for men worldwide. Despite the significant burden on both quality of life and the healthcare system this disease imposes, data on molecular analysis of biomarkers in CRC-diagnosed patients is scarce. In our study, we analyzed confirmed CRC patients from the database of the National Cancer Institute (NCI) and evaluated the presence of 4 biomarkers in tumor tissues. Altogether, 83 FFPE tumor tissues from CRC patients listed in the NCI database were analyzed for microsatellite instability status, presence of BRAF and KRAS/NRAS mutations, and neoplastic cell percentage in tissue samples. We identified 4 MSI-high samples, 39 KRAS/NRAS mutations, and 5 BRAF p.V600E mutations, with one case of coexistence of all three markers in a single tumor sample. We also evaluated possible relationships between biomarkers, their coexistence, and the age and sex of the studied population. |
