Insights into drought stress response mechanism of tobacco during seed germination by integrated analysis of transcriptome and metabolome.

Autor: Ren X; Hunan Tobacco Research Institute, Changsha, Hunan, 410004, China., Yang C; Chenzhou Tobacco Company, Chenzhou, Hunan, 423000, China., Zhu X; Hunan Agricultural University, Changsha, Hunan, 410128, China., Yi P; Changde Tobacco Company, Changde, Hunan, 415300, China., Jiang X; Hunan Agricultural University, Changsha, Hunan, 410128, China., Yang J; Hunan Tobacco Research Institute, Changsha, Hunan, 410004, China., Xiang S; Tobacco Production Technology Center, Changsha Tobacco Company, Changsha, Hunan, 410007, China., Li Y; Chenzhou Agricultural Science Research Institute, Chenzhou, Hunan, 423000, China., Yu B; Hunan Tobacco Research Institute, Changsha, Hunan, 410004, China., Yan W; Changde Tobacco Company, Changde, Hunan, 415300, China., Li X; Technology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha, Hunan, 410021, China., Li Y; Hunan Tobacco Research Institute, Changsha, Hunan, 410004, China. Electronic address: shen-ly@163.com., Hu R; Hunan Tobacco Research Institute, Changsha, Hunan, 410004, China. Electronic address: hrsh721204@163.com., Hu Z; Hunan Tobacco Research Institute, Changsha, Hunan, 410004, China. Electronic address: huzhengrong1001@163.com.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Apr; Vol. 209, pp. 108526. Date of Electronic Publication: 2024 Mar 24.
DOI: 10.1016/j.plaphy.2024.108526
Abstrakt: Drought stress inhibits seed germination, plant growth and development of tobacco, and seriously affects the yield and quality of tobacco leaves. However, the molecular mechanism underlying tobacco drought stress response remains largely unknown. In this study, integrated analysis of transcriptome and metabolome was performed on the germinated seeds of a cultivated variety K326 and its EMS mutagenic mutant M28 with great drought tolerance. The result showed that drought stress inhibited seed germination of the both varieties, while the germination rate of M28 was faster than that of K326 under drought stress. Besides, the levels of phytohormone ABA, GA19, and zeatin were increased by drought stress in M28. Five vital pathways were identified through integrated transcriptomic and metabolomic analysis, including zeatin biosynthesis, aspartate and glutamate synthesis, phenylamine metabolism, glutathione metabolism, and phenylpropanoid synthesis. Furthermore, 20 key metabolites in the above pathways were selected for further analysis of gene modular-trait relationship, and then four highly correlated modules were found. Then analysis of gene expression network was carried out of Top30 hub gene of these four modules, and 9 key candidate genes were identified, including HSP70s, XTH16s, APX, PHI-1, 14-3-3, SCP, PPO. In conclusion, our study uncovered some key drought-responsive pathways and genes of tobacco during seeds germination, providing new insights into the regulatory mechanisms of tobacco drought stress response.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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