Proteomic Analysis of Human Saliva via Solid-Phase Microextraction Coupled with Liquid Chromatography-Mass Spectrometry.

Autor:	Jiang RW; Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada., Marin LM; College of Dentistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Jaroch K; Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.; Department of Pharmacodynamics and Molecular Pharmacology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz 85-089, Poland., Zhou W; Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada., Siqueira WL; College of Dentistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Pawliszyn J; Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
Jazyk:	angličtina
Zdroj:	Analytical chemistry [Anal Chem] 2024 Apr 09; Vol. 96 (14), pp. 5363-5367. Date of Electronic Publication: 2024 Mar 27.
DOI:	10.1021/acs.analchem.4c00307
Abstrakt:	Proteomics of human saliva samples was achieved for the first time via biocompatible solid-phase microextraction (bio-SPME) devices. Upon introduction of a porogen to a conventional C18 coating, porous C18/polyacrylonitrile (PAN) SPME blades were able to extract peptides up to 3.0 kDa and more peptides than commercial SPME blades. Following Trypsin digestion, salivary proteomic analysis was achieved via SPME-LC-MS/MS. Seven endogenous proteins were consistently identified in all saliva samples via bio-SPME. Taking advantage of this strategy, untargeted peptidomics was applied for the comparison of saliva samples between healthy and SARS-CoV-2 positive individuals. The results showed clear peptidomic differences between the viral and healthy saliva samples. This proof-of-concept study demonstrates the potential of bio-SPME-LC-MS/MS for peptidomics and proteomics in biomedical applications.
Databáze:	MEDLINE
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