Metagenomic Detection and Genetic Characterization of Human Sapoviruses among Children with Acute Flaccid Paralysis in Nigeria.

Autor: George UE; African Centre of Excellence for Genomics of Infectious Diseases, Redeemer's University, Ede 232101, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede 232101, Nigeria., Faleye TOC; Center for Environmental Health Engineering, Biodesign Institute, Arizona State University, Tempe, AZ 85281, USA., De Coninck L; Laboratory of Viral Metagenomics, Department of Microbiology, Immunology and Transplantation, Rega Institute, Universiteit Leuven, 3000 Leuven, Belgium., Agbaje ST; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Ifeorah IM; Department of Medical Laboratory Science, Faculty of Health Science and Technology, College of Medicine, University of Nigeria Enugu Campus, Enugu 400241, Nigeria.; Centre for Translation and Implementation Research, University of Nigeria, Nsukka 410001, Nigeria., Onoja BA; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Oni EI; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Olayinka AO; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Ajileye TG; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Oragwa AO; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Jos, Jos 930003, Nigeria., Akinleye TE; Phytomedicine Unit, Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan 200005, Nigeria., Popoola BO; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Osasona OG; Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, Redeemer's University, Ede 232101, Nigeria.; Hospitals Management Board, Ado-Ekiti 360102, Nigeria., Olayinka OT; National Polio Laboratory, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., George OA; National Veterinary Research Institute, Vom 930101, Nigeria., Muhammad AI; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Komolafe I; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede 232101, Nigeria., Adeniji AJ; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria., Matthijnssens J; Laboratory of Viral Metagenomics, Department of Microbiology, Immunology and Transplantation, Rega Institute, Universiteit Leuven, 3000 Leuven, Belgium., Adewumi MO; Department of Virology, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria.; Infectious Disease Institute, College of Medicine, University of Ibadan, Ibadan 200212, Nigeria.
Jazyk: angličtina
Zdroj: Pathogens (Basel, Switzerland) [Pathogens] 2024 Mar 19; Vol. 13 (3). Date of Electronic Publication: 2024 Mar 19.
DOI: 10.3390/pathogens13030264
Abstrakt: Using a metagenomic sequencing approach on stool samples from children with Acute Flaccid Paralysis (AFP), we describe the genetic diversity of Sapoviruses (SaVs) in children in Nigeria. We identified six complete genome sequences and two partial genome sequences. Several SaV genogroups and genotypes were detected, including GII (GII.4 and GII.8), GIV (GIV.1), and GI (GI.2 and GI.7). To our knowledge, this is the first description of SaV infections and complete genomes from Nigeria. Pairwise identity and phylogenetic analysis showed that the Nigerian SaVs were related to previously documented gastroenteritis outbreaks with associated strains from China and Japan. Minor variations in the functional motifs of the nonstructural proteins NS3 and NS5 were seen in the Nigerian strains. To adequately understand the effect of such amino acid changes, a better understanding of the biological function of these proteins is vital. The identification of distinct SaVs reinforces the need for robust surveillance in acute gastroenteritis (AGE) and non-AGE cohorts to better understand SaVs genotype diversity, evolution, and its role in disease burden in Nigeria. Future studies in different populations are, therefore, recommended.
Databáze: MEDLINE