| Autor: |
Jameel M; Department of Theriogenology, University of Veterinary and Animal Sciences, Pakistan., Sheikh IS; Center for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Pakistan., Kakar N; Department of Natural and Basic Sciences, University of Turbat, Pakistan., Yousuf MR; Department of Theriogenology, University of Veterinary and Animal Sciences, Pakistan., Riaz A; Department of Theriogenology, University of Veterinary and Animal Sciences, Pakistan., Shehzad W; Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Pakistan., Khan D; Livestock and Dairy Development Department, Government of Balochistan, Pakistan., Iqbal M; Semen Production Unit, Livestock and Dairy Development Department, Government of Balochistan, Pakistan., Tareen AM; Department of Microbiology, University of Baluchistan, Quetta Pakistan. |
| Abstrakt: |
Reactive oxygen species at supra-physiological levels trigger oxidative stress during cryopreservation, which can be neutralised by incorporating suitable antioxidants into the semen extender medium. This study was intended to explore the effect of asiatic acid (AA) as an antioxidant in semen extender on frozen-thawed sperm quality and in vivo fertility of bull sperm. Semen was collected from Holstein Friesian bulls for 10 consecutive weeks (total ejaculates = 60). Semen was cryopreserved with a Tris citric acid egg yolk-based extender supplemented with 0 (control), 20, 40, 60, and 100 μM AA. The supplementation of the extender with 40 and 60 μM AA improved ( p < 0.05) post-thaw motility kinematics, plasma membrane integrity, acrosome integrity, sperm viability, and DNA integrity of bull sperm. Mitochondrial membrane potential was high ( p < 0.05) with 60 μM of AA concentration in extender media. The catalase activity in seminal plasma was maintained ( p < 0.05) when semen was added with 20, 40, and 60 μM of AA. The in vivo fertility was found to be significantly high with the semen extended with 60 μM AA. Conclusively, this study showed that AA supplementation in semen extender significantly improved sperm motility kinematics and cell integrity, conserved antioxidant enzyme activity, and improved in vivo fertility. |
