Cooperative dynamics of DNA-grafted magnetic nanoparticles optimize magnetic biosensing and coupling to DNA origami.

Autor:	Lak A; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), Hans-Sommer-Str. 66, Braunschweig, 38106, Germany. a.lak@tu-braunschweig.de., Wang Y; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), Hans-Sommer-Str. 66, Braunschweig, 38106, Germany. a.lak@tu-braunschweig.de., Kolbeck PJ; Soft Condensed Matter and Biophysics, Department of Physics and Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands.; Department of Physics and Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands., Pauer C; Soft Condensed Matter and Biophysics, Department of Physics and Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands., Chowdhury MS; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), Hans-Sommer-Str. 66, Braunschweig, 38106, Germany. a.lak@tu-braunschweig.de., Cassani M; International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic., Ludwig F; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), Hans-Sommer-Str. 66, Braunschweig, 38106, Germany. a.lak@tu-braunschweig.de., Viereck T; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), Hans-Sommer-Str. 66, Braunschweig, 38106, Germany. a.lak@tu-braunschweig.de., Selbach F; Department of Chemistry and Center for NanoScience, LMU Munich, 81377 Munich, Germany., Tinnefeld P; Department of Chemistry and Center for NanoScience, LMU Munich, 81377 Munich, Germany., Schilling M; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), Hans-Sommer-Str. 66, Braunschweig, 38106, Germany. a.lak@tu-braunschweig.de., Liedl T; Soft Condensed Matter and Biophysics, Department of Physics and Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands., Tavacoli J; Soft Condensed Matter and Biophysics, Department of Physics and Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands., Lipfert J; Soft Condensed Matter and Biophysics, Department of Physics and Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands.; Department of Physics and Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 1, 3584 CC Utrecht, The Netherlands.
Jazyk:	angličtina
Zdroj:	Nanoscale [Nanoscale] 2024 Apr 18; Vol. 16 (15), pp. 7678-7689. Date of Electronic Publication: 2024 Apr 18.
DOI:	10.1039/d3nr06253h
Abstrakt:	Magnetic nanoparticles (MNPs) provide new opportunities for enzyme-free biosensing of nucleic acid biomarkers and magnetic actuation by patterning on DNA origami, yet how the DNA grafting density affects their dynamics and accessibility remains poorly understood. Here, we performed surface functionalization of MNPs with single-stranded DNA (ssDNA) via click chemistry with a tunable grafting density, which enables the encapsulation of single MNPs inside a functional polymeric layer. We used several complementary methods to show that particle translational and rotational dynamics exhibit a sigmoidal dependence on the ssDNA grafting density. At low densities, ssDNA strands adopt a coiled conformation that results in minor alterations to particle dynamics, while at high densities, they organize into polymer brushes that collectively influence particle dynamics. Intermediate ssDNA densities, where the dynamics are most sensitive to changes, show the highest magnetic biosensing sensitivity for the detection of target nucleic acids. Finally, we demonstrate that MNPs with high ssDNA grafting densities are required to efficiently couple to DNA origami. Our results establish ssDNA grafting density as a critical parameter for the functionalization of MNPs for magnetic biosensing and functionalization of DNA nanostructures.
Databáze:	MEDLINE
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