Bezafibrate alleviates diabetes-induced spermatogenesis dysfunction by inhibiting inflammation and oxidative stress.

Autor:	Mu Y; Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China., Luo LB; Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China., Wu SJ; Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China., Gao Y; Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China., Qin XL; Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China., Zhao J; Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China., Liu Q; Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, 430060, China., Yang J; Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Mar 16; Vol. 10 (6), pp. e28284. Date of Electronic Publication: 2024 Mar 16 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e28284
Abstrakt:	The metabolic disorders caused by diabetes can lead to various complications, including male spermatogenesis dysfunction. Exploring effective therapeutics that attenuate diabetes mellitus (DM)-induced male subfertility is of great importance. Pharmaceuticals targeting PPARα activation such as bezafibrate have been regarded as an important strategy for patients with diabetes. In this study, we use streptozocin (STZ) injection to establish a type 1 DM mice model and use bezafibrate to treat DM mice and evaluate the effects of bezafibrate on the spermatogenic function of the DM male mice. Bezafibrate treatment exhibited protective effects on DM-induced spermatogenesis deficiency, as reflected by increased testis weight, improved histological morphology of testis, elevated sperm parameters, increased serum testosterone concentration as well as increased mRNA levels of steroidogenesis enzymes. Meanwhile, testicular cell apoptosis, inflammation accumulation and oxidative stress status were also shown to be alleviated by bezafibrate compared with the DM group. In vivo and in vitro studies, PPARα specific inhibitor and PPARα knockout mice were further used to investigate the role of PPARα in the protective effects of bezafibrate on DM-induced spermatogenesis dysfunction. Our results indicated that the protection of bezafibrate on DM-induced spermatogenesis deficiency was abrogated by PPARα inhibition or deletion. Our study suggested that bezafibrate administration could ameliorate DM-induced spermatogenesis dysfunction and may represent a novel practical strategy for male infertility. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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