Exploring Natural Immune Responses to Shigella Exposure Using Multiplex Bead Assays on Dried Blood Spots in High-Burden Countries: Protocol From a Multisite Diarrhea Surveillance Study.

Autor: Benedicto-Matambo P; School of Biomedical Sciences and Health Professions, Department of Medical Laboratory Sciences, Kamuzu University of Health Sciences, Blantyre, Malawi.; Malawi Liverpool Wellcome Programme, Blantyre, Malawi.; Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Department of Clinical Infection, Microbiology and Immunology, Liverpool, UK., Avolio LN; Department of Environmental Health & Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA., Badji H; Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia., Batool R; Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan., Khanam F; Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Munga S; Kenya Medical Research Institute, Center for Global Health Research (KEMRI-CGHR), Kisumu, Kenya., Tapia MD; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.; Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.; Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA., Peñataro Yori P; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA., Awuor AO; Kenya Medical Research Institute, Center for Global Health Research (KEMRI-CGHR), Kisumu, Kenya., Ceesay BE; Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia., Cornick J; Malawi Liverpool Wellcome Programme, Blantyre, Malawi.; Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Department of Clinical Infection, Microbiology and Immunology, Liverpool, UK., Cunliffe NA; Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Department of Clinical Infection, Microbiology and Immunology, Liverpool, UK., Garcia Bardales PF; Asociación Benéfica PRISMA, Iquitos, Peru., Heaney CD; Department of Environmental Health & Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA., Hotwani A; Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan., Ireen M; Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Taufiqul Islam M; Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Jallow O; Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia., Kaminski RW; Latham Biopharm Group, Massachusetts, USA., Shapiama Lopez WV; Asociación Benéfica PRISMA, Iquitos, Peru., Maiden V; Malawi Liverpool Wellcome Programme, Blantyre, Malawi., Ikumapayi UN; Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia., Nyirenda R; Malawi Liverpool Wellcome Programme, Blantyre, Malawi., Ochieng JB; Kenya Medical Research Institute, Center for Global Health Research (KEMRI-CGHR), Kisumu, Kenya., Omore R; Kenya Medical Research Institute, Center for Global Health Research (KEMRI-CGHR), Kisumu, Kenya., Paredes Olortegui M; Asociación Benéfica PRISMA, Iquitos, Peru., Pavlinac PB; Department of Global Health, University of Washington, Seattle, Washington, USA., Pisanic N; Department of Environmental Health & Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA., Qadri F; Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Qureshi S; Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan., Rahman N; Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Rogawski McQuade ET; Department of Epidemiology, Emory University, Atlanta, Georgia, USA., Schiaffino F; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA.; Faculty of Veterinary Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru., Secka O; Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia., Sonye C; Kenya Medical Research Institute, Center for Global Health Research (KEMRI-CGHR), Kisumu, Kenya., Sultana S; Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan., Timite D; Centre pour le Développement des Vaccins du Mali, Bamako, Mali., Traore A; Centre pour le Développement des Vaccins du Mali, Bamako, Mali., Yousafzai MT; Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan., Taufiqur Rahman Bhuiyan M; Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Jahangir Hossain M; Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia., Jere KC; Malawi Liverpool Wellcome Programme, Blantyre, Malawi.; Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Department of Clinical Infection, Microbiology and Immunology, Liverpool, UK.; School of Life Sciences & Health Professions, Department of Medical Laboratory Sciences, Kamuzu University of Health Sciences, Blantyre, Malawi., Kosek MN; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA., Kotloff KL; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.; Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.; Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA., Qamar FN; Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan., Sow SO; Centre pour le Développement des Vaccins du Mali, Bamako, Mali., Platts-Mills JA; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA.
Jazyk: angličtina
Zdroj: Open forum infectious diseases [Open Forum Infect Dis] 2024 Mar 25; Vol. 11 (Suppl 1), pp. S58-S64. Date of Electronic Publication: 2024 Mar 25 (Print Publication: 2024).
DOI: 10.1093/ofid/ofad650
Abstrakt: Background: Molecular diagnostics on human fecal samples have identified a larger burden of shigellosis than previously appreciated by culture. Evidence of fold changes in immunoglobulin G (IgG) to conserved and type-specific Shigella antigens could be used to validate the molecular assignment of type-specific Shigella as the etiology of acute diarrhea and support polymerase chain reaction (PCR)-based microbiologic end points for vaccine trials.
Methods: We will test dried blood spots collected at enrollment and 4 weeks later using bead-based immunoassays for IgG to invasion plasmid antigen B and type-specific lipopolysaccharide O-antigen for Shigella flexneri 1b, 2a, 3a, and 6 and Shigella sonnei in Shigella -positive cases and age-, site-, and season-matched test-negative controls from all sites in the Enterics for Global Health (EFGH) Shigella surveillance study. Fold antibody responses will be compared between culture-positive, culture-negative but PCR-attributable, and PCR-positive but not attributable cases and test-negative controls. Age- and site-specific seroprevalence distributions will be identified, and the association between baseline antibodies and Shigella attribution will be estimated.
Conclusions: The integration of these assays into the EFGH study will help support PCR-based attribution of acute diarrhea to type-specific Shigella , describe the baseline seroprevalence of conserved and type-specific Shigella antibodies, and support correlates of protection for immunity to Shigella diarrhea. These insights can help support the development and evaluation of Shigella vaccine candidates.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje